基质细胞衍生因子-1在视网膜培养中促进节细胞轴突再生的研究

【摘要】 目的 研究基质细胞衍生因子-1（SDF-1）在视网膜培养中促神经节细胞轴突再生的作用。设计 实验研究。研究对象 Fischer 344大鼠视网膜。方法 视网膜培养之前5天夹断大鼠视神经；培养前用多聚赖氨酸及层黏连蛋白包被培养板；培养时，把分离出来的视网膜放射状剪成8小片，每小片视网膜粘铺于已包被好的培养板孔中，每5~6块取自不同大鼠的视网膜块随机组成一组，共7组，分别置于7种不同的Neurobasal-A/B27培养基中（对照组、20 ng/ml、70 ng/ml、200 ng/ml、500ng/ml及1000 ng/ml 5个SDF-1浓度梯度处理组及SDF-1联合AMD3100阻断组）培养7天，在倒置显微镜下计数再生神经轴突的数量及长度，并对各组进行比较。主要指标 再生神经轴突的数量及长度。结果 对照组平均每个视网膜培养块的再生神经突数量及长度分别为17根及约1 mm；SDF-1促进轴突再生的作用随其浓度的增高而增强，中高浓度SDF-1组可使轴突再生增强2～3倍，而应用受体阻断剂AMD3100可使SDF-1的促再生作用减半。结论 SDF-1具有促进视网膜神经节细胞轴突再生的作用且具有浓度依赖性。（眼科，2012，21：256-260）

Stromal cell-derived factor-1 promotes axonal regeneration of retinal ganglion cells in retinal explants

Objective To investigate whether stromal cell-derived factor-1(SDF-1) has promoting effects on axonal regeneration in retinal explant.Design Experimental study.Participants Fischer 344 rats retina.Methods Optic nerve of rats were crushed 5 days before retinal explant culture,and culture plate was coated with poly-lysine and laminin before culture.Retinas were dissected out from eye cups and cut into 8 pieces followed by sticking onto the coated wells with retinal ganglion cell layer downward.Retinal pieces were incubated in neurobasal-A/B27 for 7 days before examination under an inverted fluorescent microscope.Explants were assigned to the following groups: control,SDF-1 groups with 20 ng/ml,70 ng/ml,200 ng/ml,500ng/ml,1000 ng/ml,and SDF-1 plus AMD3100 group.Main Outcome Measures Number and average length of regenerating axons.Results Average umber and length of the regenerating axons in control group were 17/explant and 1mm respectively.Promoting effects of SDF-1 was positively correlated to its concentrations.SDF-1 with middle and high concentration enhanced the axonal regeneration by 2-3 folds.The promoting effect of SDf-1 was reduced by half when adding the receptor inhibitor AMD3100.Conclusions SDF-1 has promoting effects on axonal regeneration of retinal ganglion cells in retinal explants and has dose-response.