促红细胞生成素受体抗体抑制小鼠视网膜新生血管形成

目的:观察促红细胞生成素受体抗体(erythropoietin recep-tor antibody,EpoRA)对视网膜新生血管形成的抑制作用。方法:将鼠龄为7d的C57BL/6J幼鼠置于750±20mL/L氧仓中连续饲养5d,建立高氧诱导的血管增生性视网膜病变模型。幼鼠20只右眼在建模前1d予以玻璃体内注射EpoRA 2μL作为治疗眼,左眼不注射作为对照眼。在H.E.染色的组织学切片上观察并计数突破视网膜内界膜进入玻璃体的新生血管内皮细胞核数目;用ADP酶组织化学法行视网膜铺片,观察视网膜血管改变。饲养在正常条件下的幼鼠作为正常对照组;模型幼鼠8只仅在玻璃体内注射生理盐水,作为阴性对照组。结果:在组织切片上,可见突破视网膜内界膜进入玻璃体腔内的新生血管内皮细胞核,在用EpoRA注射的右眼,明显少于未经EpoRA注射的左眼(17.20±5.42个vs23.47±8.43个;P<0.01)。ADP酶组织化学法视网膜铺片中,可见视网膜新生血管的密度和异常程度,在用EpoRA注射的右眼,明显轻于未经EpoRA注射的左眼以及阴性对照组。正常对照组未见明显异常。结论:EpoRA可抑制小鼠模型中的视网膜新生血管形成。

Inhibitory effects of erythropoietin receptor ant-body on retinal neovascularization in mice

AIM:To investigate the effects of erythropoietin receptor antibody(EpoRA)on retinal neovascularization in mice.· METHODS:Seven-day-old neonatal C57BL/6J mice were exposed to(750±20)mL/L oxygen to establish models of vascular proliferative retinopathy.The right eyes of the mice received a vitreous injection of 2μL EpoRA one day before the mice exposed to high oxygen condition.The left eyes without EpoRA injection served as controls.The effects of EpoRA on retinal neovas-cularization were evaluated by counting the endothelial nuclei of new vessels extending from the retina to the vitreous in histological sections with HE staining.The ADPase histochemical staining was used for retinal flatmount to observe the changes of retinal vessels.Neonatal mice fed in normal conditions served as normal controls;and 8 model mice only received an intravitreal injection of physiological saline served as negative control group.· RESULTS:The number of the endothelial nuclei of new vessels extending from the retina to the vitreous was significantly less in the right eyes with EpoRA injection than that in the left eyes without EpoRA injection in the model(17.20±5.42 vs 23.47±8.43,P<0.01).In the retinal flatmounts,the density and abnormality of retinal new vessels were noted much less in the right eyes with EpoRA injection compared to that of the left eyes without EpoRA injection in the model group and negative control group.No obvious changes were found in normal control group.· CONCLUSION:Retinal neovascularization can be inhibited,to some extent,by intravitreal injection of EpoRA in this mouse model.