探讨核心蛋白多糖体外抑制兔晶状体上皮细胞的转分化

目的:探讨核心蛋白多糖(decorin)对体外培养的兔晶状体上皮细胞(lens epithelial cells,LEC)分泌转化生长因子β1(transforming growth factorβ1,TGF-β1)和表达α-平滑肌肌动蛋白(α-SMA)的影响。方法:体外原代培养兔LEC,当细胞接近融合时转板培养,分别设置对照组和实验组,对照组为不用药培养12d;实验组为培养6d后加入不同浓度的decorin(10,1.0,0.1mg/L3个浓度组)继续培养6d,用ELISA法检测各组培养液上清中TGF-β1的水平,免疫细胞化学和计算机图像分析统计各组细胞中α-SMA的表达水平。结果:实验组TGF-β1水平和α-SMA水平均明显低于对照组的水平(117.9±18.1→427.2±41.3,111.7±26.7→427.2±41,236.0±28.6→427.2±41.3;15.7±8.7→97.2±26.9,16.2±9.5→97.2±26.9,54.9±29.6→97.2±26.9,P<0.01),decorin表现出明显的抑制作用;α-SMA的表达量随着TGF-β1水平的下降而下降,两者高度正相关(r=0.995,P<0.01)。结论:Decorin抑制活性TGF-β1的水平,下调α-SMA的表达。

Study to decorin inhibiting transdifferentiation of rabbits' lens epithelial cells in vitro

AIM:To investigate the effects of the decorin on secretion of transforming growth factor-β1(TGF-β1)and the expression of α-smooth muscle actin(α-SMA)in the primarily cultured rabbits’ LEC in vitro.·METHODS:Cultured rabbits’ lens epithelial cells primarily in vitro,then turned to plates when they nearly fused.Set up control group and experiment group,the control group was training 12 days without drugs,others were decorin treatment group(three concentration groups of 10,1.0,0.1mg/L)for 6 days’ treatment after training 6 days.ELISA used in each group in the culture medium supernatant of TGF-β1 levels,statistics expression of α-SMA by immunocytochemistry and computer image analysis.·RESULTS:The level of TGF-β1 and α-SMA of three experiment groups was significantly lower than the level of control group(P<0.01).And immunocytochemistry figures showed that experiment group’s hypertrophied cells and positive staining in cytoplasm was much lower than control group.It demonstrated decorin had significant inhibition effect.Moreover,α-SMA decreased accompanying to the drop of TGF-β1 and the both were highly positive correlated(r=0.995,P<0.01).·CONCLUSION:Because decorin inhibits the activity of TGF-β1 to reduced the expression of α-SMA.