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| 胎牛血清对小鼠角膜上皮细胞生长和分化的影响(英文) | |
| 引用本文： | 马小力,阙艳红,孔珺,刘汉强,张劲松.胎牛血清对小鼠角膜上皮细胞生长和分化的影响(英文)[J].国际眼科杂志,2009,9(5):817-819. |
| 作者姓名： | 马小力 阙艳红 孔珺 刘汉强 张劲松 |
| 作者单位： | 1. 中国医科大学附属第一医院眼科,中国辽宁省沈阳市,110001 2. 中国医科大学附属第一医院超声科,中国辽宁省沈阳市,110001 3. 中国医科大学附属第四医院眼科,中国辽宁省沈阳市,110001 |
| 基金项目： | 中国辽宁省教育厅基金 |
| 摘 要： | 目的:探讨胎牛血清对小鼠角膜上皮细胞生长和分化的影响。方法:分别在无血清低钙培养基(KSFM)和含100mL/L胎牛血清的KSFM中培养小鼠角膜上皮细胞。比较两种培养基中角膜上皮细胞的群体倍增(PD)。通过RT-PCR和Western blotting方法检测p63、角蛋白19以及involucrin的表达。结果:在KSFM中,小鼠角膜上皮细胞可稳定传代超过25代,但在含胎牛血清的培养基中,细胞仅能存活3代。在KSFM中,培养细胞呈典型铺路石样外观,RT-PCR和Western blotting结果显示细胞表达p63、角蛋白19以及in-volucrin。当培养基中加入胎牛血清后,细胞形态明显增大,呈扁平状; involucrin表达显著增强。结论:胎牛血清可抑制小鼠角膜上皮细胞增生、诱导其分化。 |
| 关 键 词： | 血清 角膜 上皮 细胞培养 |
Effect of fetal bovine serum on the proliferation and differentiation of murine corneal epithelial cells in vitro |
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| Xiao-Li Ma,Yan-Hong Que,Jun Kong,Han-Qiang Liu,Jin-Song Zhang.Effect of fetal bovine serum on the proliferation and differentiation of murine corneal epithelial cells in vitro[J].International Journal of Ophthalmology,2009,9(5):817-819. | |
| Authors: | Xiao-Li Ma Yan-Hong Que Jun Kong Han-Qiang Liu Jin-Song Zhang |
| Institution: | Xiao-Li Ma1,Yan-Hong Que2,Jun Kong3,Han-Qiang Liu1,Jin-Song Zhang31Department of Ophthalmology,the First Affiliated Hospital of China Medical University,Shenyang 110001,Liaoning Province,China2Department of Ultrasonics,China3Department of Ophthalmology,the Fourth Affiliated Hospital of China Medical University,China |
| Abstract: | AIM:To investigate the effect of fetal bovine serum (FBS) on the proliferation and differentiation of murine corneal epithelial cells in vitro.METHODS: Mouse corneal epithelial cells(MCEs) were cultured in serum-free low-Ca2+ medium(KSFM) and KSFM supplemented with 100mL/L FBS, respectively. Population doublings (PDs) were determined. The expressions of corneal epithelial cell markers p63, keratin 19 (K19) and involucrin were investigated by RT-PCR and Western blotting analyses. RESULTS: Cells in KSFM were ... |
| Keywords: | serum cornea epithelium cell culture |
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