| 阿司匹林对体外培养的人晶状体上皮细胞凋亡调节基因表达的影响 |
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| 引用本文： | 孔珺,孔玮,汪亚伦,张雪岩,张劲松. 阿司匹林对体外培养的人晶状体上皮细胞凋亡调节基因表达的影响[J]. 国际眼科杂志, 2009, 9(12): 2289-2291. DOI: 10.3969/j.issn.1672-5123.2009.12.009 |
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| 作者姓名： | 孔珺 孔玮 汪亚伦 张雪岩 张劲松 |
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| 作者单位： | 1. 中国医科大学附属第四医院眼科,辽宁省高校晶状体重点实验室,中国辽宁省沈阳市,110005
2. 沈阳医学院生化教研室,中国辽宁省沈阳市,110034 |
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| 基金项目： | 中国辽宁省教育厅科技攻关资助项目 |
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| 摘 要： | 目的:研究阿司匹林(Asp)对培养的人晶状体上皮细胞(humanlens epithelial cells,hLEC)Bax,Bcl-2,caspase-3表达的影响。方法:人晶状体上皮细胞系SRA01/04传代培养,MTT比色法检测Asp对bFGF诱导的hLEC增殖的影响;Western-blot方法检测Asp作用1,3,6,12,24h后凋亡相关因子Bcl-2,Bax,caspses-3蛋白的表达。结果:Asp5mmol/L可明显抑制bFGF诱导的hLEC增殖(P<0.01),且抑制作用呈浓度依赖性。Western-blot结果显示,10mmol/LAsp作用1h后Bax蛋白表达增强,随着时间的延长,表达逐渐增加,6h达到高峰,24h后恢复至基础水平;而Bcl-2的表达与其相反,Asp作用1h后Bcl-2蛋白表达明显下降,6h降到最低点,然后有上升趋势。caspase-3的表达与Bax相似,Asp作用1h后caspase-3表达增加,6h达到高峰,持续24h仍有较高的表达。结论:凋亡因子Bcl-2,Bax,caspase-3参与了Asp诱导的人晶状体上皮细胞凋亡途径。Asp通过调节凋亡基因蛋白产物的表达,诱导hLEC的凋亡。
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| 关 键 词： | 晶状体上皮细胞 阿司匹林 Bcl-2 Bax caspase-3 细胞凋亡 |
Activation of apoptosis gene in human lens epithelial cells induced by aspirin |
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| Jun Kong,Wei Kong,Ya-Lun Wang,Xue-Yan Zhang,Jin-Song Zhang. Activation of apoptosis gene in human lens epithelial cells induced by aspirin[J]. International Eye Science, 2009, 9(12): 2289-2291. DOI: 10.3969/j.issn.1672-5123.2009.12.009 |
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| Authors: | Jun Kong Wei Kong Ya-Lun Wang Xue-Yan Zhang Jin-Song Zhang |
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| Affiliation: | Jun Kong1,Wei Kong1,Ya-Lun Wang2,Xue-Yan Zhang1,Jin-Song Zhang1 1Department of Ophthalmology,the 4th Affiliated Hospital of China Medical University,Shenyang 110005,Liaoning Province,China,2Department of Biochemistry,Shenyang Medical College,Shenyang 110034 |
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| Abstract: | AIM:To investigate the possible effect of aspirin(Asp)on the proliferation of human lens epithelial cells(hLEC)and the expression of apoptosis protein Bcl-2,Bax and caspase-3 in hLEC.METHODS:Human lens epithelial cell line SRA01/04 was subcultured.MTT was used to observe the inhibition effect of Asp on the cultured cells.Western-blot was employed to detect the expression of apoptosis factors of Bcl-2,Bax and caspase-3 protein in hLEC.RESULTS:Asp treatment at different concentrations(5,10,25mmol/L)resulted i... |
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| Keywords: | Bcl-2 Bax caspase-3 |
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