新型生物活性物质尾加压素Ⅱ降眼压作用的实验研究

目的:探讨尾加压素Ⅱ(urotensinⅡ,U-Ⅱ)对兔眼压的影响。方法:将10,100pg/μL和1,10ng/μL浓度的U-Ⅱ溶液10μL分别注射到新西兰白兔眼玻璃体前部,于注射后即刻(0h),1,2,4,6,8,10,12,24,36h;2,3,4,5,6d不同时间点,用修兹眼压计行双砝码法眼压测量。结果:0.1ngU-Ⅱ组在10～12h使眼压降低17.5%(P<0.001);1ngU-Ⅱ组在8h使眼压降低33.7%(P<0.001);10ngU-Ⅱ组在6h使眼压降低54.7%(P<0.001);100ngU-Ⅱ组在6h使眼压降低56.4%(P<0.001)。四组降眼压作用分别持续24h;2,3,4d。结论:U-Ⅱ具有快速、强效、持久的降低眼压的作用,有可能成为防治青光眼的有效药物。

Experimental study of intraocular pressure decreased by new biological activity substance of Urotensin Ⅱ

AIM:To study the effect of Urotensin Ⅱ(U-Ⅱ) on intraocular pressure(IOP).METHODS:Different concentrations of U-Ⅱ(10pg/μL,100pg/μL,1ng/μL,10ng/μL) were injected into anterior vitreous body of eyes in New Zealand rabbits.Schiotz impression tonometer were used with double balances after 0,1 hour;2,4,6,8,10,12,24,36 hours;2,3,4,5,6 days.RESULTS:IOP decreased 17.5%,33.7%,54.7% and 56.4%(P<0.001) in groups of 0.1ng U-Ⅱ,1ng U-Ⅱ,10ng U-Ⅱ and 100ng U-Ⅱ after 10-12 hours,8 hours,6 hours and 6 hours respectively;The effects of IOP decreases continued until 24 hours;2,3,4 days respectively.CONCLUSION:U-Ⅱ can decrease intraocular pressure(IOP) rapidly,intensively and continuously.U-Ⅱ,the new biological activity substance may become an effective medicine to prevent and treat glaucoma.