真菌性角膜炎两种特殊染色辅助诊断的比较

目的:寻找真菌性角膜炎组织切片快速、准确、方便操作的特殊染色方式。方法:采用临床诊断疑似真菌性角膜炎或角膜溃疡的标本50例,常规固定、脱水、透明、浸蜡,进行4μm石蜡切片,常规脱蜡至水,选用高碘酸—无色品红法(PAS)和六胺银(GSM)染色法进行染色对比。结果:高碘酸—无色品红法检测阳性率为84%,六胺银染色法检测阳性率为79%。结果比较有统计学意义(P<0·05)。结论:高碘酸—无色品红染色法简便、快捷、准确,阳性率高且成本较低,优于六胺银染色法,值得推广。

Comparison between two kinds of specific stain diagnostic methods of fungal keratohelcosis

AIM:To find special staining methods which is fast, accurate and convenient operation for fungal keratitis tissue section.METHODS: Specimens of 50 cases with the clinical diagnosis of suspected fungal keratitis or corneal ulcer were selected. They were conventionally fixed, dehydrated and dipped in wax for 4μm paraffin sections, then subject to conventional dewaxing to water. Periodic acid-Schiff stain and gomori methenamine silver stain were compaired.RESULTS: Positive detection rate of periodic acid-Schiff stain was 84%, positive detection rate of gomori methenamine silver stain was 79%. Result was statistically significant (P<0.05). CONCLUSION: Periodic acid-Schiff staining method is simple, fast, accurate, high positive rate and lower cost. It is superior to gomori methenamine silver staining, and worth promoting