| 蛇毒去整合素Kistrin对正常及糖尿病兔LECs增殖的抑制作用 |
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| 引用本文： | 谭少健,韦琦,姬翔,陈金卯,何剑峰,梁皓.蛇毒去整合素Kistrin对正常及糖尿病兔LECs增殖的抑制作用[J].眼科新进展,2011,31(1):1-4. |
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| 作者姓名： | 谭少健 韦琦 姬翔 陈金卯 何剑峰 梁皓 |
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| 作者单位： | 广西医科大学第一附属医院眼科,广西壮族自治区南宁市,530021 |
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| 基金项目： | 国家自然科学基金，广西自然科学基金 |
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| 摘 要： | 目的观察高血糖对兔眼晶状体摘出术后后囊膜混浊(posterior capsular opacification,PCO)的影响,探讨蛇毒去整合素Kistrin对晶状体上皮细胞(lens epithelial cells,LECs)增殖的抑制作用。方法制作正常血糖及糖尿病兔PCO模型,随机分组:正常血糖组分为A、B两组(每组3只兔3眼),糖尿病组分为C、D两组(每组3只兔3眼),A、C组为对照组(术毕前房注入林格氏液),B、D组为用药组(术毕前房注入80mg·mL-1的Kistrin2mL)。注药后在裂隙灯显微镜下观察4组PCO发生情况及形态并计算增殖指数(PI),注药后14d时取材,检测LECs增生细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达。结果糖尿病兔PCO发生程度重于正常血糖兔;A组PI为0.25±0.05,B组0.03±0.01,C组0.86±0.04,D组0.65±0.08,A组PI明显小于C组(P=0.00),用药后B、D组PI均分别明显小于A、C组(均为P=0.01),但B组PI明显小于D组(P=0.00)。结论与正常血糖兔相比,糖尿病兔的PCO发生率较高,其LECs的增殖能力较强,Kistrin可明显抑制正常血糖及糖尿病兔的LECs增殖。
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| 关 键 词： | 后囊膜混浊 糖尿病 去整合素 增殖细胞核抗原 |
Inhibitive effect of disintegrin Kistrin on proliferation of lens epithelial cells in nor mal and diabetic rabbits |
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| TAN Shao-Jian,WEI Qi,JI Xiang,CHEN Jin-Mao,HE Jian-Feng,LIANG Hao.Inhibitive effect of disintegrin Kistrin on proliferation of lens epithelial cells in nor mal and diabetic rabbits[J].Recent Advances in Ophthalmology,2011,31(1):1-4. |
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| Authors: | TAN Shao-Jian WEI Qi JI Xiang CHEN Jin-Mao HE Jian-Feng LIANG Hao |
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| Institution: | TAN Shao-Jian,WEI Qi,JI Xiang,CHEN Jin-Mao,HE Jian-Feng,LIANG Hao From the Department of Ophthalmology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China |
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| Abstract: | Objective To observe the effects of hyperglycemia on posterior capsular opacification(PCO)after phacectomy in rabbits,and investigate the inhibitive effects of disintegrin Kistrin on lens epithelial cells(LECs).Methods The PCO models were established in normal and diabetic rabbits,then the normal rabbits with PCO were randomly divided into group A and B(3 eyes of 3 rabbits in each group),while the diabetic rabbits with PCO were randomly divided into group C and group D(3 eyes of 3 rabbits in each group).Gro... |
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| Keywords: | posterior capsular opacification diabetes disintegrin proliferating cell nuclear antigen |
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