老年性白内障晶状体上皮细胞凋亡及相关基因蛋白的表达

目的 探讨老年性白内障与晶状体上皮细胞凋亡的关系。方法透射电镜下观察老年性白内障晶状体上皮细胞的超微结构；Tunel法检测凋亡细胞百分率；并对其晶状体上皮细胞DNA进行琼脂糖凝胶电泳；免疫组化法检测P53、bcl-2在老年性白内障晶状体上皮细胞中的蛋白表达。结果 透射电镜下发现老年性白内障晶状体上皮细胞中有凋亡细胞；凋亡细胞百分率为8．4％～37．8％，琼脂糖凝胶电泳出现梯状条带；P53在老年白内障晶状体上皮细胞中蛋白表达率为16．9％～19．1％，bcl-2无蛋白表达。结论 老年性白内障的发生可能与其晶状体上皮细胞凋亡有关。

Apoptosis of lens epithelial cells in senile cataract and its related gene expression

ObjectiveTo study the relation between senile cataract and apoptotic lens epi thelial cells and the protein expression of apoptotic related genes.MethodsM orphology of lens epithelial cells from 10 cases with senile cataract and 5 norm al lens was observed under transmission electron microscopy.Apoptotic cells of l ens epithelia from senile cataract were detected with Tunel (Terminal deoxynucle ltidyl transferase mediated duTp nick and Label Ling assay),and DNA from 20 ante rior capsules of senile cataract was taken for gelose gel electrophorese .Expres sion percentage of P 53 ?bcl-2 was counted with SABC methods and DAB stain ing.ResultsApoptotic cells and apoptotic body in lens epithelial cell from s enile cataract were found and no apoptotic cell in transparent lens under transm ission electron microscopy.The apoptotic cell rate was 8 4%37 8% in se nile cataract.Genomic DNA products extracted from anterior capsules of senile ca taractous lenses showed a typical DNA ladder in agarose gel electrophoresis,and no this appearance in normal lenses.No P 53 and bcl-2 protein expression po sitive cells were seen in normal transparent lenes,but the rate of P 53 pos itive cells was 16 90%19 10% in senile cataractous lens ,there was no bcl-2 protein expression in both normal lens and senile cataractous lens.Conc lusionThere are some apoptotic cells and higher expression of P 53 in the lens epithelial cells of senile cataract ,suggesting that formation of senile c ataract is related to apoptosis of lens epithelial cells.