内毒素在Lewis鼠诱导的角膜改变——角膜平片的免疫组织化学研究

目的:探讨内毒素全身注射所致的角膜改变。方法:使用抗单核细胞、巨噬细胞和MHC-Ⅱ类抗原阳性细胞的单克隆抗体,用标准的ABC方法于内毒素注射前、后制备的角膜平片上进行免疫组织化学染色。结果:发现在正常角膜中央区实质内有散在分布的巨噬细胞,越近角膜缘分布越密集,MHC-Ⅱ类抗原阳性细胞则仅存在于角膜缘;内毒素注射后,中央区角膜单核巨噬细胞增多,这些细胞于角膜实质内发生了一系列形态学改变,MHC-Ⅱ类抗原阳性细胞仅见于注射后早期的中央区角膜内皮面。结论:内毒素诱导的角膜中巨噬细胞的增多可能是机体应激状态下的重要防御机制,角膜实质中MHC-Ⅱ类抗原的缺如则对维持角膜局部免疫微环境的稳定性有重要意义。眼科学报1996;12:70—74。

Changes of the Cornea Induced by Lipopolysaccharide in Lewis Rats

Purpose: To investigate changes of the cornea induced by lipopolysaccharide (LPS). Methods:Immunohistochemical study using monoclonal antibodies to monocytes, macrophages (ED1,ED2) and MHC class II antigen (OX6) was performed on the corneal wholemounts obtained from normal lewis rats and those after LPS injection. Results:Macrophages were noted to be present in whole cornea with a gradually decreased cell density from limbus to central part in normal lewis rats. However, major histocompatibility complex class II antigen (MHC class II )-positive cells were only distributed at limbus. Footpad injection of LPS induced an increase of monocytes and macrophages in whole cornea and a dramatical changes of these cells morphologically. MHC class I -positive cells were only and shortly noted on the surface of the cornea endothelium at early stage after LPS injection. Conclusion: LPS-induced increase of macrophages in the cornea may be an important defense mechanism in response to LPS challenge. On the other hand, the absence of MHC class II -positive cells both in the normal and in the inflamed cornea may be contributed to the stability of immunological microenvironment within this tissue. Eye. science 1996; 12 : 70- 74.