视网膜母细胞瘤SO—Rb70细胞系的建立及液氮冻存和复苏

目的:建立新的人视网膜母细胞瘤细胞株,对其进行冻存和复苏,为临床、科研提供Rb细胞库。方法:用精确优化的取材,不经离心,直接通过洗涤吹打细胞后接种,并根据慢冻速融的细胞冻存原则,定期收集建株的Rb_(70)细胞作液氮冻存及复苏。结果:成功建立SO-Rb_(70)细胞株,共冻存8批细胞,进行了6次复苏实验,4次获成功。结论:经液氮冻存的人Rb_(70)细胞,复苏后生长良好,保持了细胞原有的生物活性及细胞特征,为Rb的深入研究提供了丰富的实验研究材料。眼科学报1998;14:80—82。

The Establishment, Freezing-state Preservation and Resuscitation of Retinoblastoma SO-Rb_(70) Cell Line

Purpose: To establish a new cell line of human retinoblastoma (Rb),frozen and resuscitated to provide a Rb cell reservoir for clinical and experimental research. Methods :The screening cells were washed and blown directly without centrifugation and then inoculated. Baesd on the cell-frozen principle, the Rb cell line was collected and established, then frozen in liquid nitrogen and resuscitated. Results: SQ-Rb70cell line was. established successfully. 8 groups of cells were frozen. The resuscitation experiment was carried out for 6 times ,4 times being successful. Conclusion: The preserved human Rb cells in liquid nitrogen grew normally after resuscitation with the maintenance of previous bioactivities and characteristics. This research helped to provide abundant material for further study of Rb. Eye Science 1998; 14:80-82.