首页 | 本学科首页   官方微博 | 高级检索  
检索        

地塞米松诱导人小梁细胞bcl—2基因的表达
引用本文:卓业鸿,葛坚.地塞米松诱导人小梁细胞bcl—2基因的表达[J].眼科学报,1998,14(4):187-189.
作者姓名:卓业鸿  葛坚
作者单位:中山医科大学中山眼科中心,中山医科大学中山眼科中心,中山医科大学中山眼科中心,中山医科大学中山眼科中心,中山医科大学中山眼科中心 广州 510060,广州 510060,广州 510060,广州 510060,广州 510060
基金项目:国家自然科学基金(编号39770789,39800163),广东省科委基金(编号980112)
摘    要:目的:探讨地塞米松对小梁细胞bcl-2基因表达的影响。方法:应用组织块培养的方法建立人小梁细胞培养。取第三代小梁细胞培养于载玻片上,含10~(-7)mol/L地塞米松的培养液作用6小时、12小时、24小时后,应用LSAB观察bcl-2基因表达的情况。结果:地塞米松作用6小时后可见bcl-2蛋白阳性细胞,且随时间的延长,阳性细胞有所增多。结论:地塞米松可诱导小梁细胞bcl-2基因表达,可能在激素性青光眼发病中起着一定的作用。眼科学报1998;14:187~189。

关 键 词:地塞米松  小梁细胞  bcl-2基因

Expression of bcl-2 Gene in Human Trabecular Cells Induced by Dexamethasone
Yehong Zhuo,Jian Ge,Minkai Linjianliang Zheng,Yanyan Li Zhongshan Ophthalmic Center,Sun Yat-sen University of Medical Sciences,Guangzhou ,China.Expression of bcl-2 Gene in Human Trabecular Cells Induced by Dexamethasone[J].Eye Science,1998,14(4):187-189.
Authors:Yehong Zhuo  Jian Ge  Minkai Linjianliang Zheng  Yanyan Li Zhongshan Ophthalmic Center  Sun Yat-sen University of Medical Sciences  Guangzhou  China
Institution:Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Abstract:Purpose:To investigate the influence of dexamethasone on expression of bcl-2 gene in human trabecular cells. Methods: The trabecular specimens from human eyes were cultured in vitro and the cultured cells of the third generation on slides were immersed in culture solution containing 10-7M dexamethasone for 6 hours, 12 hours and 24 hours respectively. The expression of bcl-2 gene was studied with LSAB. Results: The positive cells of the bcl-2 protein could be identified after dexamethasone effected for 6 hours and the positive cell increased with time going on. Conclusion :The expression of bcl-2 gene can be induced by dexamethasone, it may be related with glaucomatic pathogenesis. Eye Science 1998 ; 14 ; 187 - 189.
Keywords:dexamethasone  trabecular cells  bcl-2 gene
本文献已被 CNKI 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号