| The Study on Culture and Ultrastructure of Glaucomatous Trabecular Cells in Vitro |
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| 引用本文： | Jian Ge Minkai Lin 等. The Study on Culture and Ultrastructure of Glaucomatous Trabecular Cells in Vitro[J]. 眼科学报, 1998, 14(3): 134-137 |
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| 作者姓名： | Jian Ge Minkai Lin 等 |
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| 作者单位： | Jian Ge，Minkai Lin，Jianliang Zheng，Yehong ZhuoZhongshan Ophthalmic Center，Sun Yat-sen University of Medical Sciences，Guangzhou 510060，China |
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| 摘 要： | Purpose : To establish the culture system of human glaucomatous trabecular cells in vitro and study their ultrastructures.Methods : The trabecular specimens from trabeculectomy were cultured in vitro and passaged 3 times, then identified. Moreover, the glaucomatous cells were observed with electron microscope while compared with the normal ones.Result: Cultured human glaucomatous trabecular cells were obtained. The ultrastructure of the cells showed the decrease in vilious project, coated vesicle and lysosomal inclusion. Conclusion : The establishment of human glaucomatous trabecular cells culture in vitro made the culture system more perfect. The morphologic changes might be related to the abnormal functions of human trabecular meshwork cells. Eye Science 1998; 14 : 134 - 737.
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| 关 键 词： | 青光眼 小梁细胞 离体培养 超微结构 |
The Study on Culture and Ultrastructure of Glaucomatous Trabecular Cells in Vitro |
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| Jian Ge,Minkai Lin,Jianliang Zheng,Yehong ZhuoZhongshan Ophthalmic Center,Sun Yat-sen University of Medical Sciences,Guangzhou ,China. The Study on Culture and Ultrastructure of Glaucomatous Trabecular Cells in Vitro[J]. Eye science, 1998, 14(3): 134-137 |
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| Authors: | Jian Ge Minkai Lin Jianliang Zheng Yehong ZhuoZhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China |
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| Affiliation: | Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China. |
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| Abstract: | Purpose : To establish the culture system of human glaucomatous trabecular cells in vitro and study their ultrastructures.Methods : The trabecular specimens from trabeculectomy were cultured in vitro and passaged 3 times, then identified. Moreover, the glaucomatous cells were observed with electron microscope while compared with the normal ones.Result: Cultured human glaucomatous trabecular cells were obtained. The ultrastructure of the cells showed the decrease in vilious project, coated vesicle and lysosomal inclusion. Conclusion : The establishment of human glaucomatous trabecular cells culture in vitro made the culture system more perfect. The morphologic changes might be related to the abnormal functions of human trabecular meshwork cells. Eye Science 1998; 14 : 134 - 737. |
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| Keywords: | glaucoma trabecular cell culture ultrastructure |
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