高糖对视网膜Meller细胞VEGF，EPO和EPO RmRNA表达的影响

目的：观察高糖条件下VEGFmRNA，EPOmRNA和EPORmRNA在体外培养的Muiler细胞中的表达情况。方法：胰蛋白酶将新生小鼠视网膜组织吹打消化后，制成单细胞悬液，体外培养Muller细胞，RT．PCR测定高糖条件下视网膜Mailer细胞VEGF，EPO和EPOR基因的表达。结果：成功获得视网膜Muller细胞，传代后90％以上的细胞呈兔抗鼠谷氨酰胺合酶（GS）染色阳性。Muller细胞VEGFmRNA，EPO mRNA和EPORmRNA在高糖条件下表达升高，且呈浓度依赖性（P〈0．05），但糖浓度50mmol／L组较40mmol／L组差异无统计学意义，无明显时间依赖性。结论：Muller细胞在高糖条件下VEGF，EPO，EPOR的表达增加。

Effect of high levels of glucose expressions of VEGF, EPO and EPOR retinal Muller cells on in

AIM ：To observe the expressions of vascular endothelial growth factor （VEGF）, erythropoietin （EPO）, erythropoietin receptor（ EPOR） mRNA in different levels of glucose in the retinal Muller cells of mice in vitro. METHODS： MUller cells of new-born mice were cultured with enzyme digestion. Muller cells were separated and purified by blowing and striking method. The expressions of VEGF mRNA, EPO mRNA, and EPOR mRNA were detected by RT-PCR. RESULTS： Retinal MUller cells were cultured successfully, 90% of which were positively stained by glutamine synthetase （GS）. The expressions of VEGF mRNA, EPO mRNA and EPOR mRNA in retinal Muller cells were significantly enhanced in the high glucose group which increased obviously（ P〈 0. 05）, but the difference between 50mmol/L group and the 40mmol/L group did not have statistical significance or time dependence. CONCLUSION： With glucose concentration elevating, VEGF, EPO, EPOR expression of cultured retinal Muller cells increased.