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卵裂期胚胎可溶性人类白细胞抗原G的表达及其与胚胎发育的关系
作者姓名:Wang Q  Zhuang GL  Zhou CQ  Li J  Zhong YP  Xu YW  Zhang MF  Deng MF
作者单位:510080,广州,中山大学附属第一医院生殖医学研究中心
基金项目:广东省卫生厅基金资助项目(B2001023)
摘    要:目的观察卵裂期胚胎可溶性人类白细胞抗原G (soluble human leukocyte antigen G, sHLA-G)的表达,并探讨sHLA-G的表达与胚胎发育的关系.方法采用免疫组化标记法,检测177个卵裂期胚胎sHLA-G的表达情况.结果 177个卵裂期胚胎中,101个卵裂期胚胎有sHLA-G蛋白表达,sHLA-G的总表达率为57.1%(101/177).其中双原核受精卵发育形成的卵裂期胚胎sHLA-G的表达率为66.2 %(90/136),三原核受精卵发育形成的卵裂期胚胎sHLA-G的表达率为26.8 %(11/41),两者比较,差异有统计学意义(P<0.01).双原核受精卵发育形成的1级卵裂期胚胎sHLA-G 的表达阳性率为64.3%(18/28),2级卵裂期胚胎为91.7%(66/72),3级卵裂期胚胎为16.7%(6/36).3者比较,差异有统计学意义(P<0.01).三原核受精卵发育形成的Ⅰ级卵裂期胚胎sHLA-G的阳性率为88.9%(32/36),与双原核受精卵发育形成的Ⅰ级卵裂期胚胎比较,差异有统计学意义(P<0.01).双原核受精卵发育形成的≤4个细胞的胚胎sHLA-G的表达率为56.7%(34/60), 5个细胞及6个细胞的胚胎为67.9%(36/53),7个细胞及8个细胞的胚胎为87.0%(20/23),3者比较,差异无统计学意义(P>0.05).结论卵裂期胚胎有sHLA-G蛋白表达;且sHLA-G表达与胚胎发育有关.

关 键 词:HLA-G  胚胎发育  表达率  卵裂期  原核  可溶性人类白细胞抗原G  G蛋白  受精  阳性率
修稿时间:2004年5月27日

Expression of soluble human leukocyte antigen G and its relationship to embryo development
Wang Q,Zhuang GL,Zhou CQ,Li J,Zhong YP,Xu YW,Zhang MF,Deng MF.Expression of soluble human leukocyte antigen G and its relationship to embryo development[J].Chinese Journal of Obstetrics and Gynecology,2005,40(2):112-115.
Authors:Wang Qiong  Zhuang Guang-lun  Zhou Can-quan  Li Jie  Zhong Yi-ping  Xu Yan-wen  Zhang Ming-fan  Deng Ming-fen
Institution:Reproductive Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Abstract:OBJECTIVE: To find out the expression of soluble human leukocyte antigen G (sHLA-G) and its relationship to the cleavage embryo development. METHODS: One hundred and seventy-seven day 3 cleavage embryos were detected for sHLA-G by immunohistochemistry. RESULTS: sHLA-G was detected in 57.1% cleavage embryos. The positive rate of sHLA-G in cleavage embryos developed from dipronucleate fertilized eggs was 66.2% (90/136), that developed from tripronucleate fertilized eggs was 26.8% (11/41). There was significant difference between these two groups (P < 0.01). The positive rate of sHLA-G in the grade 1 cleavage embryos developed from dipronucleate fertilized eggs was 64.3% (18/28), that in the grade 2 cleavage embryos developed from dipronucleate fertilized eggs was 91.7% (66/72), that in the grade 3 cleavage embryos developed from dipronucleate fertilized eggs was 16.7% (6/36), there were significant differences between these different embryo grades (P < 0.01), and the intensity of sHLA-G had negative relationship with the embryo grades (r = -0.503). The positive rate of sHLA-G in the first class cleavage embryos developed from tripronucleate fertilized eggs was 88.9% (32/36), that from dipronucleate fertilized eggs was 64.3% (18/28). There was significant difference in these two groups (P < 0.01). The intensity of sHLA-G in cleavage embryos developed from tripronucleate fertilized eggs was higher than that from dipronucleate fertilized eggs. The positive rate of sHLA-G in the cleavage embryos developed from dipronucleate fertilized eggs whose cell number was less than 4 was 56.7% (34/60), that from dipronucleate fertilized eggs whose cell number ranged from 5 to 6 was 67.9% (36/53), and that from dipronucleate fertilized eggs whose cell number ranged from 7 to 8 was 87.0% (20/23). There were no significant differences in these three groups. The intensity of sHLA-G had no significant difference between embryos with different cell number (P > 0.05), but it had relationship with the cell number (r = 0.267). CONCLUSION: Cleavage embryos express sHLA-G which is related with the embryo development.
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