控制性卵巢刺激中黄体期添加雌激素对小鼠子宫内膜组织胞饮突变化和白血病抑制因子表达的影响

目的 研究控制性卵巢刺激(COS)中黄体期添加雌激素对子宫内膜容受性的影响,探讨COS中不同黄体期支持方案对小鼠子宫内膜中胞饮突发育情况、白血病抑制因子(LIF)表达的影响.方法 将正常雌性昆明小鼠随机分为A组(单纯COS)、B组(COS+黄体酮)、C组(COS+黄体酮+雌激素)和D组(自然周期,未应用药物)共4组,扫描电镜下观察各组小鼠妊娠第3～5天子宫内膜胞饮突的发育情况;采用免疫组化方法 检测LIF蛋白在各组小鼠妊娠第3～5灭子宫内膜中的表达情况.结果 (1)B、C、D组小鼠妊娠第3天子宫内膜出现发育中的胞饮突,妊娠第4天出现完全发育的胞饮突,妊娠第5天出现退化的胞饮突;A组小鼠妊娠第3天可见少量完伞发育的胞饮突,妊娠第4天可见退化的胞饮突.(2)妊娠第3～5大小鼠子宫内膜LIF蛋白表达水平的平均值,C组(138.5±20.3)、D组(143.1±19.0)均明显高于A组(103.2±5.0),差异均有统计学意义(P<0.05),C、D组妊娠第4天LIF蛋白表达均最强,呈强阳性;B组表达水平(123.5±10.8)高于A组,但低于C、D组,差异也均有统计学意义(P<0.05),B组妊娠第4天LIF蛋白表达最强,呈阳性;A组小鼠妊娠第3天LIF蛋白表达最强,呈弱阳性.A组妊娠第3天小鼠与B组、C组和D组妊娠第4天小鼠子宫内膜中LIF蛋白表达水平之间两两比较,差异均有统计学意义(F=55.76,P<0.01).结论 COS中黄体期支持时添加雌激素能改善小鼠子宫内膜中胞饮突的发育及LIF蛋白的表达,从而增加子官内膜容受性.

Effect of estradiol supplementation during the luteal phase on mouse endometrial expression of leukaemia inhibitory factor and pinopodes in controlled ovarian stimulation cycles

Objective To study the effect of estradiol supplementation during the luteal phase on mouse endometrial expression of leukaemia inhibitory factor and pinopodes in controlled ovarian stimulation cycles.Methods Female mice were randomly divided into four groups:group Acontrolled ovarian stimulation(COS)group],group B(COS group with progesterone for luteal-phase-support),group C(COS group with progesterone and estradiol for luteal-phase-support),and group D of natural cycle group.Pinopodes were investigated by scanning electronic microscopy(SEM)in the uterine endometrium of pregnant mice on pregnancy days(pa)3-5.LEukaemia inhibitory factor(LIF)protein Was determined by immunohistochemistry in the uterine endometrium of pregnant mice on pd 3-5.Results (1)In groups B,C,and D,there were small developed pinopodes in the endometrial surface of pregnant mouse on day 3;there were large fully developed pinopodes in endometrial surface,which Was smooth with well defined borders resembling a mushroom on day 4.The regressing pinopodes were observed on day 5.In group A,there were small developed pinopodes in endometrial surface of pregnant mouse on day 3.The regressing pinopodes were seen on day 4.(2)In the pregnant mice of groups C and D,the level of LIF protein on days 3-5 ( 138.5±20. 3,143.1±19. 0) was significantly higher than group A ( 103. 2 ± 5.0, P < 0. 05 ), and strong immunostaining of LIF protein was found on day 4 of gestation. In group B, the level of LIF protein on days 3-5 ( 123.5±10. 8)was significantly higher than group A (P <0. 05), but significantly lower than groups C and D ( P <0. 05 ). Strong immunostaining of LIF protein was found on day 4 of gestation. In group A, weak immunostaining of LIF protein peaked on day 3 of gestation. In groups B, C, and D, the level of LIF protein on day 4 was significantly higher than group A on day 3 ( F = 55.76, P < 0. 01 ). Conclusions Estradiol supplementation during the luteal phase can improve the expression of LIF and pinopodes in mouse endometrium in controlled ovarian stimulation cycles and redress the harmful effect on implantation window by COS. Therefore, estradiol supplementation can improve the endometrial receptivity.