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玻璃化冷冻人早期胚胎的效果观察
作者姓名:Zhu GJ  Jin L  Zhang HW  Li YF  Wei YL  Hu J
作者单位:430030,武汉,华中科技大学同济医学院附属同济医院妇产科生殖中心
摘    要:目的探讨玻璃化冷冻人早期胚胎的临床应用价值。方法采用由5.5mol/L乙二醇及1.0mol/L蔗糖制成的冷冻保护液,于0.25ml冷冻麦管中,冷冻经体外受精一胚胎移植第2天或第3天后剩余的胚胎;对新鲜周期胚胎移植后未妊娠的胚胎,至少3个月或3个月以上施行胚胎复苏,观察胚胎复苏后的临床妊娠效果。结果共行胚胎复苏219例,957个胚胎,胚胎复苏后存活691个,胚胎存活率为72.2%(691/957);移植胚胎178例,临床妊娠35例,临床妊娠率为19.7%(35/178)。35例中,分娩16例,流产6例,继续妊娠13例。结论玻璃化冷冻法简便,胚胎存活率高,是一种可用于临床的人早期胚胎冷冻的方法。

关 键 词:玻璃化冷冻  胚胎  受精  体外  玻璃化冷冻法  人早期胚胎  临床妊娠率  临床应用价值  胚胎存活率  mol/L  胚胎移植
收稿时间:2004-10-23
修稿时间:2004年10月23

Vitrification of human cleaved embryos in vitro fertilization-embryo transfer
Zhu GJ,Jin L,Zhang HW,Li YF,Wei YL,Hu J.Vitrification of human cleaved embryos in vitro fertilization-embryo transfer[J].Chinese Journal of Obstetrics and Gynecology,2005,40(10):682-684.
Authors:Zhu Gui-jin  Jin Lei  Zhang Han-wang  Li Yu-feng  Wei Yu-lan  Hu Juan
Institution:Reproductive Center, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Abstract:Objective To assess the outcome of thawing human cleaved embryos from cryopreservation by vitrification. Methods A total of 957 day 2 or day 3 embryos from 219 patients were thawed after vitrification with ethylene glycol 5.5 solution and 0. 25 ml straw between Jan 2003 and Jun 2005,514 embryos were recovered and transferred in 178 patients. Results The survival rate of thawing embryos and the clinical pregnancy rate after transfer was 72. 2% and 19. 7% respectively. Twenty-two healthy babies were born from 16 deliveries, including 12 girls and 10 boys, 6 pregnancies ended in miscarriage and another 13 are ongoing. Conclusion Vitrification method is an alternative for cryopreservation of human cleaved embryos because of high effectiveness, convenience and good cost efficiency.
Keywords:Vitrification  Embryo  Fertilization in vitro
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