补肾益气和血方中药对胚泡着床障碍小鼠子宫内膜表面胞饮突表达的影响

目的　观察补肾益气和血方中药对胚泡着床障碍小鼠子宫内膜表面胞饮突表达的影响。方法　选择 8～ 12周龄昆明小鼠 ,于妊娠第 4天 (Pd4 )晨 ,皮下注射米非司酮 ,制成胚泡着床障碍模型。然后将妊娠小鼠随机分为正常组、模型组和治疗组 ,治疗组从Pd1开始灌服中药 (补肾益气和血方 ) ,正常组和模型组则给予等量生理盐水灌服 ,模型组和治疗组于Pd4晨皮下注射米非司酮 ,正常组则注射等量生理盐水。采用扫描电镜观察各组小鼠在Pd4晚 2 1时 30分～ 2 2时 (第 1时间点 ,T1)和Pd5上午 9时 30分～ 10时 (第 2时间点 ,T2 ) ,共两个时间点子宫内膜表面胞饮突的表达。结果　模型组妊娠率 ( 2 5 % )较正常组 ( 90 % )显著降低 ,差异有显著性 (P <0 0 5 ) ,模型组平均着床胚泡数为 ( 7 7± 1 3)个 ,正常组为 ( 14 7± 1 4 )个 ,两组比较 ,差异有极显著性 (P <0 0 0 1) ;治疗组的妊娠率 ( 5 5 % )及平均着床胚泡数 ( 12 3± 0 8)个 ]均较模型组显著增加 (P <0 0 5 ,P <0 0 1)。T1时正常组子宫内膜表面表达大量发育中的胞饮突 ,至T2时则表达大量发育完全的胞饮突 ;T1时模型组子宫内膜发育不同步 ,胞饮突仅在局部少量表达 ,至T2时则表达完全消失 ;T1时治疗组子宫内膜表面表达大量发育中的胞饮突 ,与正常组相比略延

Effect of Bushenyiqihexue recipe on the expression of endometrial pinopodes in blastocyst implantation dysfunctional mice

OBJECTIVE: To observe the effect of Bushenyiqihexue recipe on the expression of endometrial pinopodes in blastocyst implantation dysfunctional mice. METHODS: To induce blastocyst implantation dysfunctional mice model, Kunming mice were injected subcutaneously with mifepristone on day 4 of pregnancy. First, Kunming mice were randomly assigned to the control group, the model group and treated group postcoitally. Every morning from day 1 of pregnancy (Pd1), the treated group was administered the Bushenyiqihexue recipe decoction, while the model group and control group were administered saline orally. On Pd4, the model and treated groups were injected subcutaneously with mifepristone diluted in propylene glycol, while the control group was injected with saline only. The expression of endometrial pinopodes between 21:30 and 22:00 on Pd4 (T1) and between 9:30 and 10:00 on Pd5 (T2) was determined by scanning electron microscopy. RESULTS: Compared with control group (90%, 14.7 +/- 1.4), pregnancy rate (25%) and mean implanted blastocysts (7.7 +/- 1.3) in model group were remarkably reduced (P < 0.05, P < 0.001). Compared with the model group, pregnancy rate (55%) and mean implanted blastocysts (12.3 +/- 0.8) in treated group were significantly improved (P < 0.05, P < 0.01). At T1, abundant developing pinopodes were expressed in endometrial surface in control group, whereas abundant fully developed pinopodes expressed at T2. At T1, only a few pinopodes were expressed locally in endometrial surface in model group, while completely disappeared at T2. At T1, endometrial surface in treated group expressed a lot of developing pinopodes which lagged behind slightly that of the control group in time, whereas many fully developed pinopodes expressed at T2. CONCLUSIONS: Decreased pinopodes expression at T1 and complete loss expression of pinopodes at T2 seem to be the reasons for blastocyst implantation dysfunction induced by mifepristone. It is suggested that Bushenyiqihexue recipe could improve pinopodes expression in endometrial surface, eventually better uterine receptivity and improve the blastocyst implantation.