促性腺激素释放激素激动剂对化疗损伤卵巢功能保护作用的实验研究

目的研究促性腺激素释放激素激动剂(GnRHa)对注射环磷酰胺(CTX)大鼠的卵巢功能的保护作用。方法80只Fischer344大鼠分为4组,即空白对照组、GnRHa组、CTX组及联合治疗组,每组20只,分别接受生理盐水、CTX、GnRHa和CTX+GnRHα注射,采用酶联免疫吸附实验和化学发光法测定各组大鼠卵泡刺激素(FSH)和雌二醇浓度的变化,在实验开始后第60、90天,各组分别处死一半大鼠,观察卵巢重量、卵泡数量及卵泡直径的变化。结果实验第90天,CTX组大鼠血清雌二醇和FSH浓度分别为(148·3±16·5)pmol/L和(16·90±1·90)U/L,明显高于联合治疗组的(91·8±9·9)pmol/L和(7·60±0·30)U/L,两组分别比较,差异有统计学意义(P<0·05);CTX组大鼠卵巢重量为(37·0±3·0)mg,低于联合治疗组的(71·0±5·0)mg,两组比较,差异有统计学意义(P<0·05);CTX组大鼠卵泡总数为(550±50)个,而联合治疗组为(1250±160)个,两组比较,差异有统计学意义(P<0·05)。结论GnRHa能够降低CTX对大鼠卵巢功能的损伤,从而保护大鼠卵巢储备功能。

Study of the prevention of chemotherapy-induced ovarian damage by gonadotropin-releasing hormone agonist in rat model

OBJECTIVE: To study the effect of gonadotropin-releasing hormone agonist (GnRHa) against cyclophosphamide (CTX) induced gonado toxicity in female rats. METHODS: Eighty Fischer 344 rats were divided into four groups which received normal saline, CTX, GnRHa and CTX + GnRHa respectively. We measured the serum estradiol and follicle-stimulating hormone (FSH) concentration monthly, and respectively killed half of the rats two months and three months later to compare the weight of the ovary, the numbers of the follicle and the mean diameter of the follicles. RESULTS: After three months, in the rats in the CTX group, the serum estradiol and FSH were (148.3 +/- 16.5) pmol/L and (16.90 +/- 1.90) U/L respectively. They were significantly higher than (91.8 +/- 9.9) pmol/L and (7.60 +/- 0.30) U/L in the rats in the combination therapy group (P < 0.05). The follicle number of rats in the CTX group was 550 +/- 50 and the follicle number in the combination group was 1250 +/- 160. There was significant difference between two groups (P < 0.05). Between rats in the control and combination therapy groups, there was no obvious difference in all the indexes we examined. CONCLUSIONS: Combination of the CTX and GnRHa in the rat model could decrease the CTX-induced damage to the ovarian function.