自体骨与同种异体骨治疗兔桡骨骨缺损的实验对比研究

目的:探讨采用同种异体骨治疗兔桡骨骨缺损疗效的实验研究。方法:将12只健康成年新西兰大白兔作为同种异体骨供体,28只作为实验受体。按美国组织库标准制备同种异体骨。将受体实验动物双侧桡骨中段切除10 mm,制成骨缺损模型。受体实验动物右侧桡骨骨缺损内植入自体髂骨骨粒(对照组);左侧取等量同种异体骨粒同法植入(实验组)。于术后2、4、8、12周,对骨缺损修复组织内OPG蛋白的表达检测采用免疫组织化学方法。术后12周,检测两组骨小梁相对体积、骨小梁数量和骨密度。结果:术后两组未见感染现象发生。术后2、4周实验组OPG蛋白表达低于对照组,差异有统计学意义(P<0.05)。术后8、12周两组OPG蛋白表达差异无统计学意义。术后12周两组骨小梁相对体积、骨小梁数量和骨密度比较,差异无统计学意义。结论:同种异体骨具有较好的组织相容性,其骨性愈合过程与自体骨移植相似,并且来源广泛,能够避免因自体骨移植带来的诸多并发症,可以成为治疗骨缺损的重要材料。

The experimental comparison study of rabbit radius defects treated with autogenous and autogenic bone

Objective:To investigate experimental study of curative effects with allogeneic autogenic bone in rabbit radius defects.Methods: 12 New Zealand rabbits were provided allogeneic bone under the standard of American Association of Tissue Bank.28 experimental rabbits.were created 10 mm bone defect model in the bilateral radii.In the left side,allogeneic bone were used to repair bone defect(experimental group),equal capacity autogenous iliac bone was used in the right side(control group).Immunohistochemical meth...