表没食子酸酯诱导卵巢癌SKOV3细胞凋亡及机制的研究

目的：探讨表没食子酸酯（EGCG）对人卵巢癌SKOV3细胞凋亡的影响及其作用机制。方法：SKOV3细胞分为4组，即阴性对照组（以正常培养液培养）、EGCG低浓度组（1 μmol/L EGCG）、EGCG中浓度组（10 μmol/L EGCG）和EGCG高浓度组（100 μmol/L EGCG）。应用四甲基偶氮唑盐（MTT）比色法测定各组SKOV3细胞的增殖抑制率，Hoechs染色观察细胞核凋亡，电镜观查细胞形态，蛋白质印迹（Western blot）检测Caspase-3表达。结果：细胞增殖抑制实验结果显示，EGCG低、中、高浓度组SKOV3细胞的增殖率为（81.33±3.71）%、（67.00±7.21）%和（54.67±3.84）%，与阴性对照组（101.00±3.06）%比较差异具有统计学意义（P＜0.05）。Hoechst33258染色结果显示，EGCG低、中、高浓度组凋亡细胞比例分别为（16.77±2.48）%、（24.33±3.70）%和（39.67±4.11）%，与阴性对照组（2.00±1.45）%]相比差异均有统计学意义（Ｐ＜０．０５）。电镜观察结果显示，随着EGCG作用浓度的增加，SKOV3细胞超微结构损害程度逐渐明显。此外，EGCG可以升高Caspase-3表达水平。结论：EGCG通过增强Caspase-3表达诱导卵巢癌SKOV3细胞凋亡。

EGCG Induce the Apoptosis of Ovarian Cancer SKOV3 Cells and Its Mechanism

Objective:To investigate the effect and mechanism of EGCG on human ovarian cancer SKOV3 cells′apoptosis. Methods:SKOV3 cells were divided into four groups. Negative control group:cultured with normal culture medium;EGCG low dose group:cultured with 1μmol/L EGCG;EGCG middle dose group:cultured with 10μmol/L EGCG;EGCG high dose group:cultured with 100μmol/L EGCG. Cell proliferation inhibition rate of each group of SKOV3 cells were test by MTT assay, nuclei apoptosis were checked by Hoechs staine, cells morphology were investigated by electron microscope, Caspase-3 expression was detected by Western-blot. Results:Cell proliferation inhibition test results showed that the inhibition rate of EGCG low, medium and high concentration group on SKOV3 cells was (81.33±3.71)%, (67.00±7.21)%and (54.67±3.84)%, the difference was statistically significant (P<0.05) compared with the negative control group (101.00 ±3.06)%. Hoechst33258 staining results showed that, apoptosis cell percentage of EGCG low, medium and high concentration group was (16.77 ±2.48)%, (24.33±3.70)%and (39.67±4.11)%, the difference was statistically significant (P<0.05) compared with the negative control group (2.00±1.45)%]. Electron microscopy showed that with the concentration of EGCG elevated, the damage on ultrastructure of SKOV3 cell became clear. In addition, EGCG could raised Caspase-3 expression levels. Conclusions:EGCG induced ovarian cancer SKOV3 cells apoptosis by enhancing the expression of Caspase-3 gene.