丙戊酸钠诱导孤独症大鼠模型复制的实验研究

目的探讨丙戊酸钠诱导孤独症大鼠模型的复制方法。方法采用妊娠12．5d孕鼠腹腔内注射丙戊酸钠（600mg／kg），所产仔鼠为模型组大鼠；对照组孕鼠注射同等剂量的生理盐水，所产仔鼠为对照组大鼠。观察并测试模型组、对照组仔鼠的生长发育、社交及非社交行为、交流和重复刻板动作。结果与对照组大鼠相比，模型组大鼠出现生长发育迟缓、非社交性行为增加和社交性行为减少、对社交性气味花费时间减少及重复刻板动作增加（P〈0．05）。结论应用丙戊酸钠复制孤独症大鼠模型，操作简单易行，成功率高。

Replication of sodium valproate-induced animal model of autism

Objective To investigate the replication method of sodium valproate-induced animal model of autism. Methods Female rats received a single intraperitoneal injection of sodium valproate（600 mg/kg） on the 12.5 day after concep- tion in the experiment,the pups as model group;and control females were injected with physiological saline at the same time,the pups as control group. Sodium valproate was dissolved in saline at a concentration of 250 g/L. Observe and test the physical development,social interaction,communication and stereotyped, repetitive patterns of behaviors of the pups. Test physical de- velopment and social interaction and communication and physical contact with companion. Results Compared with control group,model group rats showed lower body weight, delayed open eyes; attenuated coordination of direction and swimming, lower levels of following and push-crawl and higher levels of self-grooming, and lower levels of sniffs of the cotton swab of rat urine. Conclusion The operation of replicate sodium valproate-induced animal model of autism is simple. The success rate of rat model is high.