| IL-2基因转移对卵巢癌细胞系SKOV3粘附性的影响 |
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| 引用本文： | 张震宇,郎景和.IL-2基因转移对卵巢癌细胞系SKOV3粘附性的影响[J].现代妇产科进展,1999,8(1):22-24. |
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| 作者姓名： | 张震宇 郎景和 |
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| 作者单位： | 北京军区总医院妇产科(张震宇)，北京协和医院妇产科(郎景和) |
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| 摘 要： | 目的：研究ＩＬ－２基因转移对卵巢癌ＳＫＯＶ３细胞系粘附性的影响。方法：细胞对塑料基质、细胞基底膜成分的粘附实验，流式细胞术测定ＣＤ４４Ｈ的表达。结果：ＥＤＴＡ介导的细胞分离实验表明，ＳＫＯＶ３／ＩＬ－２细胞对塑料基质的粘附能力明显下降（Ｐ＜０．０１）；对基质及基底膜成分的粘附实验表明，ＳＫＯＶ３／ＩＬ－２细胞对Ⅳ型胶原和ｍａｔｒｉｇｅｌ的粘附能力下降，但对ＬＮ、ＦＮ的粘附能力无明显改变；粘附分子ＣＤ４４Ｈ的表达：ＳＫＯＶ３、ＳＫＯＶ３／Ｎｅｏ、ＳＫＯＶ３／ＩＬ－２组细胞粘附分子ＣＤ４４Ｈ阳性细胞百分率分别为７６．２％、６６．７％和３７．８％，ＳＫＯＶ３／ＩＬ－２组明显降低（Ｐ＜０．００１）。结论：ＩＬ－２基因修饰可使卵巢癌细胞系ＳＫＯＶ３粘附能力下降。
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| 关 键 词： | 白细胞介素2基因 卵巢癌 基因治疗 粘附性 |
The effect of human interleukin 2 gene transfer on the adhesive ability of ovarian cancer cell line SKOV3. |
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| Zhang Zhenyu Lang Jinghe. Dept.of Gynecology and Obstetrics,General Hospital of Beijing Military Command, Peking Union Medical College Hospital.The effect of human interleukin 2 gene transfer on the adhesive ability of ovarian cancer cell line SKOV3.[J].Current Advances In Obstetrics and Gynecology,1999,8(1):22-24. |
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| Authors: | Zhang Zhenyu Lang Jinghe Deptof Gynecology and Obstetrics General Hospital of Beijing Military Command Peking Union Medical College Hospital |
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| Institution: | Zhang Zhenyu Lang Jinghe. 1Dept.of Gynecology and Obstetrics,General Hospital of Beijing Military Command,100700 2 Peking Union Medical College Hospital |
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| Abstract: | Objective:To evaluate the adhesive ability of ovarian cancer cell line SKOV3 and the IL 2 gene modified cells.Methods:EDTA induced cell detachment and cell adhesive ability to LN,FN,collagen Ⅳ and matrgel were performed.The percentage of CD44H positive cells was detected by FCM. Results:Cell adhesion tests showed that the hIL 2 modified SKOV3 cells adhered less effectively to plastic substance,collagen Ⅳ and matrigel,but there were no significant difference to LN and FN.The percentage of CD44H positive cells decreased from 76.2% of parental SKOV3 cells to 37.8% of hIL 2 gene modified cells.Conclusion:IL 2 gene modification can significantly decrease the adhesive ability of SKOV3 cells. |
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| Keywords: | Interleukin 2 Gene Ovarian neoplasms gene therapy Adhesiveness |
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