胸腺基质淋巴细胞生成素基因多态性与子痫前期的相关性研究

目的探讨胸腺基质淋巴细胞生成素(TSLP)位点rs1898671、rs2289278,rs2289276及rs764916基因多态性与妊娠妇女子痫前期发生的相关性。方法收集2012年11月至2013年6月中国医科大学附属盛京医院分娩的孕妇384例。其中病例组181例,对照组203例,病例组又分为子痫前期轻度组(97例)和子痫前期重度组(84例)。选取TSLP4个位点(rs1898671,rs2289276,rs2289278 and rs764916),利用基质辅助激光解析电离飞行时间质谱(MALDITOF MS)技术分析该基因的4个位点。结果各临床资料在病例组与对照组比较中差异均有统计学意义(P0.05),子痫前期轻度组与对照组比较中除年龄外其他各临床资料比较,差异均有统计学意义(P0.05),子痫前期重度组与对照组以及子痫前期轻度组比较中各临床资料差异均有统计学意义(P0.05)。位点rs1898671等位基因频率在病例组与对照组中差异有统计学意义(P=0.043,OR=2.682,95%CI 1.020~7.054)。结论 TSLP位点rs1898671基因多态性与子痫前期的发病可能具有相关性。等位基因T为子痫前期的危险性等位基因。

Correlation between the TSLP gene polymorphism and preeclampsia.

Abstract： Objective To explore the correlation between gene polymorphism of the sites of thymic stromal lymphopoietin （TSLP）（rs1898671， rs2289276， rs2289278 and rs764916）and occurrence of preeclampsia in pregnant women.Methods A total of 384 pregnant women who visited Shengjing Hospital of China Medical University from November 2012 to June 2013 were enrolled.Total 181 women with preeclampsia were selected，including 97 women with mild preeclampsia and 84 women with severe preeclampsia，and 203 normal pregnant women were in cotrol group.The polymorphism of four sites（rs1898671， rs2289276， rs2289278 and rs764916） was analyzed by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS）. Results The clinical characteristics was significantly different between case and control groups (P<0.05). The clinical characteristics were significantly different between mild preeclampsia and control groups except for maternal age (P<0.05). There was an obvious statistical significance between severe preeclampsia and control groups. The allele frequency and genotypes frequency of rs1898671 was significantly different between the case group and control group（P=0.043， OR=2.682， 95% CI 1.020～7.054）.Conclusion Rs1898671 of the TSLP gene is probably associated with preeclampsia.The T allele is the risk allele.