重度子痫前期患者胎盘组织中Survivin和Caspase-3表达

目的探讨重度子痫前期患者胎盘组织中Survivin和Caspase-3的表达,以及其与早发型重度子痫前期的发生,发展关系。方法采用免疫组织化学染色方法检测20例正常妊娠孕妇(对照组)、40例重度子痫前期患者(重度子痫前期组,其中早发型20例,晚发型20例)的胎盘组织进行Survivin和Caspase-3的定位,并用计算机图像分析系统进行定量分析比较。结果重度子痫前期组与对照组比较,Survivin在胎盘滋养层细胞的细胞浆及部分细胞核中的表达低于对照组,差异有统计学意义(P0.05),Caspase-3在胎盘滋养层细胞的细胞膜、细胞浆中的表达高于对照组,差异有统计学意义(P0.05)。且两者呈负相关关系(r=-0.84,P=0.0001)。早发型重度子痫前期与晚发型重度子痫前期比较,Survivin显著降低,而Caspase-3表达增加。结论Survivin的表达降低,Caspase-3的表达升高可能与重度子痫前期的发生有关。

Expression of Survivin and Caspase-3 in placenta of severe preeclampsia

Objective To study the expression of Survivin and Caspase-3 in placenta of severe preeclampsia, thus to detect their effects in the pathogenesis of the early-onset severe preeclampsia.Methods Twenty normal pregnant women （control group） and forty women with preeclampsia were recruited in this study. According to the onset time, preeclampsia group were divided into two groups：early-onset preeclampsia group （〈34 weeks, 20 patients） and later-onset preeclampsia group （≥34 weeks, 20 patients）. The expression of Survivin and Caspase-3 in placenta in both groups were determined by immunohistochemistry. Statistic analysis was done by image analysis system.Results The level of Survivin in placental villus trophoblast cytoplasm of pre-eclampsia group was significantly lower than that of control group （P〈0.05）.The level of Caspase-3 in the placental villus trophoblast and cytoplasm membrane of pre-eclampsia group was significantly higher than that of control group（P〈0.05）.Compared with the later- onset, early-onset group had lower Survivin expression and higher Caspase-3 expression. Conclusion Survivin and Caspase-3 may participate in the pathogenic process of pre-eclampsia.