mmu-miR-141在早孕小鼠胚胎着床前、后子宫内膜中的表达及作用

目的:探讨mmu-miR-141在早孕小鼠胚胎着床前、后子宫内膜组织中的表达及作用。方法:荧光定量PCR(FQ-PCR)及原位杂交检测早孕小鼠胚胎着床前、后子宫内膜mmu-miR-141的表达;MTT和流式细胞术检测孕第4日(d 4)子宫内膜基质细胞被mmu-miR-141抑制剂作用72 h后其细胞活力和细胞凋亡情况。结果:mmu-miR-141在小鼠胚胎着床后(d 6)子宫内膜组织中的表达明显低于着床前(d 4)(P<0.05),且表达于基质细胞;其表达下调能使基质细胞增殖活力下降(P<0.05),细胞凋亡增加(P<0.01)。结论:mmu-miR-141通过调控子宫内膜基质细胞的增殖或凋亡,在早孕小鼠胚胎着床前、后的子宫内膜组织中发挥重要的作用。

Expression and Role of mmu-miR-141 in Endometrium of Early PregnantMouse during Embryo Implantation

Objective: To investigate the expression and the role of mmu-miR-141 in endometrium of early pregnant mouse during embryo implantation. Methods: Fluorescence quantitative PCR (FQ-PCR) and in situ hybridization were used to detect the expression of mmu-miR-141 in endometrium of early pregnant mouse. The flow cytometry (FCM) and MTT were applied to detectet viability and apoptosis of endometrial stromal cells on pregnant day 4 affected by mmu-miR-141 inhibitor for 72 h. Results: The mmu-miR-141 expression in endometrial tissue after implantation (d 6) was obviously lower than that before implantation (d 4)(P<0.05). Moreover, the mmu-miR-141 has been demonstrated in the stromal cells and its down-regulated expression can decrease stromal cell proliferation significantly (P<0.05) and increase rate of apoptosis (P<0.01). Conclusion: The mmu-miR-141 plays an important role in endometrium of early pregnant mouse before and after the embryo implantation through regulating the proliferation of endometrial stromal cells and its apoptosis.