环氧司坦在妊娠大鼠上的药理学和组织学研究

环氧司坦(epostane,Epo)为3β-羟甾脱氢酶抑制剂,在口服96mg/kg的剂量下,可使大鼠终止妊娠,血浆孕酮水平下降。肌肉注射hCG(150 IU/鼠)不能拮抗Epo的止孕作用。光镜下发现Epo使妊娠大鼠的子宫内膜增殖、弯曲,上皮呈假复层,核分裂;大部分黄体细胞核固缩或消失,细胞质出现空泡。大鼠于妊娠d 10口服Epo后,电镜下发现d 12时黄体细胞排列不规则,细胞间隙增宽,脂滴增多,电子密度下降;d 16时滑面内质网减少。结果表明Fpo作用于黄体,其溶黄体作用可能是Epo止孕作用的原因之一。

Pharmacological and Histological Studies of Epostane in Pregnant Rats

Epostane, a 3β-hydroxysteroid dehydrogenase inhibitor, terminated pregnancy with decreased plasma levels of progesterone in pregnant rats at a single oral dose of 96mg/kg. An intramuscular injection of hCG (150 IU/rat) did not nullify its interceptive effect. After epostane was given orally on d10 of pregnancy in rats, the endometrium and luteal cells were examined under light and electron microscopes. Light-microscopically, the endometrium was found to be proliferous and curled. Epithelial cells were pseudostratified with divided nuclei. Most granulosa luteal cells were irregularly arranged, karyopyknosis occured, nuclei disappeared and the cytoplasm was vacuolated. Electron-microscopically, on d12 of pregnancy, luteal cells became smaller, irregular gaps enlarged, lipid droplets increased in number and the electron density decreased, while on d16 of pregnancy, the smooth endoplasmic reticulum decreased in number. Results show that epostane is acting at the level of the corpus luteum and its luteolytic effect might be one of the causes of its interceptive action.