精子线粒体膜电位与男性精液参数和体质量指数的相关性研究

目的探讨精子线粒体膜电位(MMP)与精子常规参数及体质量指数(BMI)的相关性。方法在本院辅助生殖科行精液分析的男性患者,禁欲3~7 d后,以手淫方式获取精液样本。通过计算机辅助精液分析仪检测精液常规,改良巴氏染色检查精子形态,JC-1染色后经流式细胞仪评估MMP。结果与对照组(56.68%±11.13%)相比,弱精子组MMP(41.24%±9.71%)显著降低。MMP与BMI呈显著负相关(r=-0.25,P0.01),与精子总活力(r=0.63,P0.01)、前向运动力(r=0.64,P0.01)及正常精子形态率(r=0.37,P0.01)呈显著正相关,而与年龄、精液量、精子浓度和精子数量其余参数的相关性均无统计学意义。结论精液中精子MMP是评估精子功能的重要指标,对临床综合分析男性不育症因素具有一定的参考意义。

Correlation between mitochondrial membrane potential of sperm and semen paremeters and male obesity

Objective To investigate the relationship among sperm mitochondrial membrane potential (MMP),semen parameters and body mass index (BMI).Methods Semen samples were obtained by masturbation after 3-7 d of sexual abstinence from all men in semen collect room of Shanghai Ninth People's Hospital.Conventional semen analyses were performed by computer-assisted sperm analysis (CASA),and sperm morphology was analyzed by modified Papanicolaou staining.Spermatozoa were stained by JC-1 to evaluate the MMP by flow cytometry.Results Sperm MMP of asthenozoospernic group (41.24％ ± 9.71％) was significantly lower than that in the control (56.68％ ± 11.13％).MMP was negatively correlated with BMI (r=-0.25,P＜0.01),but had instead a positive correlation with sperm total motility (r=0.63,P＜0.01),progressive motility (r=0.64,P＜0.01) and normal sperm morphology (r=0.37,P＜ 0.01),respectively.And there was no statistically different correlation among MMP,age,volume of semen,sperm concentration,and sperm count.Conclusion Sperm MMP was an important index to evaluate the function of sperm,which has a certain reference value for clinical comprehensive analysis of male infertility.