前列腺上皮特异性Ets转录因子(PDEF)表达与激素非依赖性前列腺癌临床分期分级的相关性研究

Correlation Study on PDEF Expression and Clinical Stage, Gleason Grade of Hormone-independent Prostate Cancer

Objective：To investigate the correlation between prostate epithelium-specific Ets transcrip-tion factor（PDEF） expression and Gleason grade and clinical stage in hormone-independent prostate can-cer（PCa）.Methods：Thirty androgen-dependent prostate cancer specimens（group A） and 30 androgen-independent prostate cancer specimens（group B） were collected in this retrospective study.PDEF gene and protein expression was detected with immunohistochemical SP method,RT-PCR semi-quantitative method and Western blotting method.The correlation between PDEF expression and Gleason grade,clinical stage was analyzed.Results：Immunohistochemical results showed that PDEF positive expression rate was 70%（21/30） in group A,while that was 96.7%（29/30） in group B（P0.05）.PDEF positive expression rate in cases with ≤7 points in Gleason grade was significantly lower than that in the cases with 7 points（64.3% vs 93.7%,P0.01）.PDEF positive expression rate in the cases with T2 in clinical stage was significantly higher than that in the cases with ≤ T2（86.8% vs 54.5%,P0.01）.RT-PCR experiments further confirmed that there was a significant difference in PDEF mRNA expression between group A and group B（P0.01）.PDEF mRNA expression in the cases with ≤7 points in Gleason grade was significantly lower than that in the cases with 7 points（P0.01）.PDEF mRNA expression in the cases with T2 was significantly higher than that in the cases with ≤ T2（P0.01）.Western blotting result showed that PDEF protein expression was as the same as PDEF mRNA.Conclusion：PDEF was highly expressed in androgen-independent prostate cancer specimens,and further up-regulated with the increase of clinical stage and Gleason grade,indicating that PDEF might involve in the occurrence and development of PCa,and thus PDEF might be a target gene in the study and treatment of hormone-independent PCa.