| 着床研究模型:离体胚泡与子宫内膜“共培养” |
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| 引用本文： | 刘承权,沈淑人,戴茂征.着床研究模型:离体胚泡与子宫内膜“共培养”[J].生殖与避孕,1989(4). |
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| 作者姓名： | 刘承权 沈淑人 戴茂征 |
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| 作者单位： | 上海市计划生育科学研究所
(刘承权，沈淑人)，上海市计划生育科学研究所(戴茂征) |
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| 摘 要： | 本工作用小鼠和豚鼠的离体胚泡与子宫内膜共培养方法,为研究着床与抗着床机理提供了一个体外研究模型。子宫内膜取自妊娠小鼠第5天上午8时前和妊娠豚鼠第4天下午,分别与小鼠或豚鼠胚泡共培养。经培养2～3天后可见胚泡从透明带逸出并附着于子宫内膜,部分植入内膜。
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| 关 键 词： | 子宫内膜 胚泡 共培养 |
A MODEL FOR IMPLANTATION:CO-CULTURE OF EMBRYOS AND ENDOMETRIA |
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| Liu Cheng-quan,et al.A MODEL FOR IMPLANTATION:CO-CULTURE OF EMBRYOS AND ENDOMETRIA[J].Reproduction and Contraception,1989(4). |
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| Authors: | Liu Cheng-quan |
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| Institution: | Shanghai Institute of Planned Parenthood Research |
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| Abstract: | An attempt has been made to develop an in vitro system for the study of implantation and anti-implantation process in mice and guinea pigs. Endometria tissues were obtained from day 5 of pregnancy in mice and day 4 of pregnancy in guinea pigs. Blastocysts were collected from mice in the early morning of Day 5 of pregnancy and in guinea pigs on day 4 of pregnancy. Blastocysts were washed and allotted to the endometrial cultures. Incubation continued for 4～5 days.During the first 2 days of culture the endometrial epithelium grew to confluence and a well developed basal lamina was restored. Blastocysts in coculture with endometria, hatched from the zona pellucida, attached and some of them invaded the endometria. |
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| Keywords: | Embryo Endometrium Blastocyst Co-culture |
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