| 损毁弓状核致大鼠肥胖型无排卵机制探讨 |
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| 引用本文： | 张洪芹,张连双,李雅娜,时彦,赵伟. 损毁弓状核致大鼠肥胖型无排卵机制探讨[J]. 实用妇产科杂志, 2009, 25(6) |
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| 作者姓名： | 张洪芹 张连双 李雅娜 时彦 赵伟 |
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| 作者单位： | 滨州医学院组织胚胎学教研室,山东,烟台,264003 |
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| 基金项目： | 山东省教育厅科技计划项目 |
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| 摘 要： | 目的:探讨注射谷氨酸钠损毁弓状核致大鼠肥胖后对其不孕的影响.方法:给新出生雌性Wistar大鼠皮下注射谷氨酸钠(实验组),连续5天,以损毁其弓状核建立肥胖模型.对照组注射等量0.9%氯化钠液,连续5天.最终筛选出实验组肥胖不孕大鼠模型20只和对照组大鼠20只.观察两组神经肽Y(NPY)、瘦素受体(leptin R)及促性腺激素释放激素(GnRH)在其下丘脑弓状核的表达变化;并测定血清雌二醇(E2)、睾酮(T)、瘦素(leptin)、卵泡刺激素(FSH)、黄体生成素(LH)的水平.结果:在实验组大鼠中,血清E2、T、leptin水平较对照组明显升高(P<0.05),FSH、LH水平较对照组明显降低(P<0.05);下丘脑弓状核NPY表达较对照组增强(P<0.05),而leptin R及GnRH免疫阳性胞体及纤维表达较对照组减弱(P<0.05).结论:损毁弓状核致大鼠肥胖由于神经内分泌-代谢失调而引起GnRH水平降低,导致下丘脑-垂体-卵巢轴功能失调性无排卵而引起不孕.
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| 关 键 词： | 损毁弓状核 肥胖 不孕 大鼠 |
The Mechanism of Anovulation in Obesity Rats Infertility after the Arcuate Nucleus Lesion |
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| ZHANG Hongqin,ZHANG Lianshuang,LI Yana,et al. The Mechanism of Anovulation in Obesity Rats Infertility after the Arcuate Nucleus Lesion[J]. Journal of Practical Obstetrics and Gynecology, 2009, 25(6) |
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| Authors: | ZHANG Hongqin ZHANG Lianshuang LI Yana et al |
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| Affiliation: | Binzhou Medical College;Yantai Shandong 264003;China |
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| Abstract: | Objective:To investigate the effects of obesity inducing infertility after the arcuate nucleus lesion by injection Glutamic Sodium in rats.Methods:Subcutaneous injection of Glutamic Sodium to 40 newborn female Wistar rats for 5 continuous days to create the obesity model by arcuate nucleus lesion,and another 40 rats were chosen as control.The expressions of neusopeptide Y(NPY),leptin receptor,and gonadotrophin-releasing hormone(GnRH) in arcuate nucleus of hypothalamus were observed by immunohistochemistry,a... |
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| Keywords: | Arcuate nucleus lesion Obesity Infertility Rats |
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