SMAD2/3蛋白在沙鼠脑缺血再灌注及氟桂利嗪干预后脑内表达的研究

目的 研究smad2/3在沙鼠脑缺血再灌注脑内表达及氟桂利嗪干预后对其表达的影响.方法 35只沙鼠随机分假手术组、缺血再灌注组和氟桂利嗪治疗组3组,采用夹闭双侧颈总动脉制作沙鼠脑缺血再灌注模型,于缺血10 min再灌注1 d、3 d、7 d各时间点处死动物,用免疫组化方法检测各组沙鼠脑内smad2/3的表达情况.结果 假手术组沙鼠脑内smad2/3弱阳性表达,而缺血再灌注1 d,3 d,7 d后沙鼠脑内呈阳性或强阳性表达,两者比较差异有统计学意义(P<0.01);氟桂利嗪治疗组smad2/3的表达低于缺血再灌注组,2组比较差异有统计学意义(P<0.05).结论 沙鼠脑内有smad2/3表达,脑缺血再灌注后,脑内smad2/3表达上调;氟桂利嗪干预后,其表达下调.

Expression of smad2/3 protein and the effects of flunarizine on its expression in brain tissue following transient cerebral ischemic reperfusion in gerbils

Objective To study the expression of smad2/3 protein and the effects of flunarizine on the its expression in braln tissue following transient cerebral ischemie reperfusion in gerbils. Methods A cerebral ischemia-reperfusion model in gerbils was established by clamping both common carotids. Thirty-five gerbils were randomly divided into three groups, sham operation group, cerebral ischemia-reperfusion group and flunarizine treatment group. The expression of smad2/3 protein in brain tissue was detected by immunohistochemistry technique. Results Experimental results revealed that smad2/3 protein was expressed in neuroeyte in 35 gerbil brain. Compared with sham operation group, the expression of smad2/3 protein in neurecytes of cerebral isehemia-reperfusion group was evidently increased at the lst day, 3rd day and 7th day (P <0. 01). Compared with cerebral ischemia-reperfusion group, the expression of smad2/3 protein in neurecytes of gerbils in flunarizine treatment group was evidently decreased at these time point (P < 0.05). Condusions Smad2/3 protein was expressed in nettrcvytes of gerbils. Expression of smad2/3 protein in neuroeytes of gerbils was evidently increased following cerebral ischemic reperfusion, and its expression in flunarizine treatment group was evidently decreased.