载脂蛋白A5基因多态性及血清水平与代谢综合征的相关性研究

目的探讨载脂蛋白A5（ApoA5）基因多态性及其血清水平与代谢综合征的相关性。方法选择代谢综合征（MS）患者100例（代谢综合征组）和健康人100例（对照组）。用ELISA法检测两组的载脂蛋白A5血清浓度，采用PCR—RFLP法测定两组的ApoA5—1131T〉C基因多态性，并用全自动分析仪测空腹血糖（FPG）、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）及白细胞、血红蛋白、血小板、肝肾功能。结果代谢综合征组与对照组比较：ApoA5血清浓度明显降低（96．68±18．09）n∥mlvs（128．32±23．78）μg／ml，P〈0．01]，TG明显升高（2．35±1．07）mmol／LVS（1．62±1．13）mmol／L，P〈0．01）]，而且代谢综合征组的ApoA5—1131C基因型频率明显高于对照组（30％vs16．5％，P〈0．05）。结论代谢综合征患者的ApoA5血清浓度明显降低，ApoA5—1131C与代谢综合征有相关性。

Study on the relationship between metabolic syndrome and Apolipoprotein A5 (ApoA5) gene polymorphism and serum concentration

Objective To explore the relationship between metabolic syndrome and ApoA5 gene polymorphism and serum concentration. Methods 100 patients with metabolic syndrome(MS group) and 100 healthy people(control group) were enrolled in this study. The ApoA5 serum concentration of two groups were measured by using enzyme-linked immunosorbent assay(ELISA), the genotypes of ApoA5-1131T＞C polymorphism were analyzed by polymerase chain reaction-restriction fragment length polymorphism, and fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) and white blood cell, hemoglobin,platelet, liver and kidney function were measured. Results MS group was compared with control group. In MS group, the ApoA5 serum concentration was significantly lower(96.68±18.09)ng/ml vs (128.32±23.78)ng/ml,P＜0.01], while the triglycerides levels were obviously higher(2.35±1.07)mmol/L vs (1.62±1.13)mmol/L,P＜0.01], and ApoA5-1131C allele frequency was higher than that in control group (30% vs 16.5%,P＜0.05). Conclusions The ApoA5 serum concentration in metabolic syndrome was decreased, and ApoA5-1131C was associated with metabolic syndrome.