经典型霍奇金淋巴瘤EB病毒感染和p16蛋白表达的相关性研究

目的探讨EB病毒在经典型霍奇金淋巴瘤（CHL）中的感染和p16蛋白表达的关系。方法应用EBER-1寡核苷酸探针以原位杂交方法检测80例CHL组织中的EBV编码的小RNA；用免疫组化方法检测p16蛋白表达。结果80例CHL患者中，男52例，女28例，男女之比为1．86：1，其中52例CHL男性患者中，EBER-1阳性率63．46％；28例CHL女性患者中，EBER-1阳性率39．29％，两者比较具有统计学意义（Х^2=4．298，P=0．038）。儿童和老年人中CHL患者共45例，其EBV表达阳性率75．56％，多于成年人的28．57％，两者比较差异具有统计学意义（Х^2=20．13，P=0．000）。EBER．1阳性表达定位在R—s细胞核上，其阳性表达率为55．00％，各亚型CHL的EBV潜伏感染检出率分别是：混合细胞型（MC）71．79％，淋巴消减型（LD）42．85％，淋巴细胞为主型（LP）47．06％，结节硬化型（NS）29．41％，且MC型明显高于NS型（Х^2=8．787，P=0．003）；p16蛋白阳性表达定位在R—S细胞的细胞核和细胞浆，其总的阳性表达率为45％，各亚型阳性率分别为：IJP47．05％，MC48．71％，NS35．29％，LD42．85％；p16蛋白与EBER-1之间呈负相关（r=-0．242，P〈0．05）。结论CHL发生可能与EBV感染有关，且p16蛋白的高表达为协助诊断CHL提供了依据。

The relationship of Epstein-Barr virus infection and expression of p16 protein in classic Hodgkin's lymphoma

Objective To study the relationship between EBV infection of classic Hodgkin＇s lyphoma and expression of p16 protein. Methods EBER-1 and p16 protein expression in 80 cases of CHL were studied with EBER-1 oligonucleotide probe and immunohistochemistry respectively. Results 80 cases of CHL were enrolled in this study, including male 52 cases, 28 cases of female, male to female ratio of 1.86 ： 1. The positive rate of EBER-1 in female was 39. 29% and in male was 63.46% ,which have statistically significant difference （ Х^2 = 4. 298, P = 0. 038）. The positive rate of EBER-1 in children and the older was significantly higher than that in adult （Х^2 = 20. 13, P = 0. 000）. EBER-1 positive expression located in the R-S nuclei which positive expression rate was 55.00%, including mixed cell type （MC） 71.79%, lympho- cyte depletion type （LD） 42. 85%, lymphocyte predominance （LP） 47.06% and nodular sclerosis type （NS） 29.41%. MC type was significantly higher than NS （ X^2 = 8. 787, P = 0. 003 ） ;p16 was noted in the nucleus and cytoplasm. The total positive expression rate was 45%, and subtype positive rates were 47.05% （ LP）, 48.71% （ MC）, 35.29% （NS） and 42. 85% （LD）. There was a negative correlation between p16 protein and EBER-1 （ r = -0. 242, P 〈 0. 05）. Conclusions CHL may be related to EBV latent infection and it can be considered as potential markers to identify HRS cells in diagnosis.