| HLA-B常用分型方法的应用分析 |
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| 引用本文： | 傅茜,曾文涛,王东,邓素雄,陈连周,王长希.HLA-B常用分型方法的应用分析[J].中国医师杂志,2005(Z1). |
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| 作者姓名： | 傅茜 曾文涛 王东 邓素雄 陈连周 王长希 |
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| 作者单位： | 中山大学附属第一医院外科 广东广州510080
(傅茜，曾文涛，王东，邓素雄，陈连周)，中山大学附属第一医院外科 广东广州510080(王长希) |
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| 摘 要： | 目的探讨血清学、序列特异性引物-聚合酶链反应(PCR-SSP)方法和多聚酶链反应寡聚核苷酸探针杂交(PCR-SSOP)技术3种方法在HLA-B位点分型的应用价值。方法研究样本30份,为等待肾移植供、受者外周血。血清学方法采用单克隆抗体一步法、PCR-SSP采用微量SSP法,PCR-SSOP为反向杂交。结果所有样本用3种方法进行HLA-B分型均获得成功,分型结果血清学方法与PCR-SSP不相合率为13%,PCR-SSOP与PCR-SSP不相合率为3%。结论血清学分型方法误差率较高、分辨率低,但成本不高,最为快速简便。PCR-SSP和PCR-SSOP分型方法特异性好,准确率高。SSP适用于少量标本,而SSOP虽耗时长,却可同时检测大批量的样本。
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| 关 键 词： | HLA-B 血清学方法 PCR-SSP PCR-SSOP |
Utility Analysis of the Useful Methods for HLA-B Typing |
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| FU Qian,ZENG Wen-tao,WANG Dong,et al..Utility Analysis of the Useful Methods for HLA-B Typing[J].Journal of Chinese Physician,2005(Z1). |
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| Authors: | FU Qian ZENG Wen-tao WANG Dong |
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| Institution: | FU Qian,ZENG Wen-tao,WANG Dong,et al. The First Affiliated Hospital of Sun-yat-sen University,Guangzhou 510080,China |
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| Abstract: | Objective To evaluate the practical value of Serology, PCR-SSP and PCR-SSOP for HLA-B typing. Methods A total of 30 samples, the blood of patients and donors waiting kidney transplantation, were used in the study. HLA-B typing was performed by one-step monoclonal antibody typing, micro PCR-SSP typing and PCR-SSOP reverse hybridisation. Results All samples were successfully typed by three methods. The difference between serological and PCR-SSP typing was 13%. The difference between PCR-SSOP and PCR-SSP typing was 3%. Conclusion Serology is high discrepancy rate and low-resolution, but cheap, simple and rapid. PCR-SSP and PCR-SSOP typing are high specific and accuracy. SSP is suitable for several samples, and SSOP is for lots of samples simultaneously although it needed long time. |
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| Keywords: | HLA-B Serology PCR-SSP PCR-SSOP |
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