慢性鼻-鼻窦炎炎性黏膜中黏蛋白MUC5AC的表达

目的 检测慢性鼻-鼻窦炎筛窦黏膜中黏蛋白MUC5AC的表达.方法 采用免疫组化和荧光定量巢式RT-PCR分别检测32例慢性鼻-鼻窦炎和7例正常对照组筛窦黏膜中MUC5AC的表达.结果 MUC5AC蛋白表达在正常对照组的筛窦黏膜中的灰度值为159.72±14.14,在CRS的筛 窦黏膜中的灰度值为115.80±31.58,两者之间差异具有统计学意义（t=3.57,P＜0.01）;MUC5ACmRNA在CRS筛窦黏膜中的定量表达（35.80±19.74）×105拷贝/μg]高于正常对照组（4.66±2.47）×105拷贝/μg],两组之间的差异具有统计学意义（t=4.12,P＜0.01）.结论 MUC5AC在慢性鼻-鼻窦炎筛窦黏膜中表达上调,可能是慢性鼻-鼻窦炎黏液过量分泌的重要因素.

The expression of MUC5AC in sinus mucosa of chronic rhinosinusitis

Objective To investigate the expression of MUC5AC in human sinus mucosa and compare the expression of MUC5AC mRNAs in normal and chronic sinus mucosa. Methods Thirty two chronic ethmoid sinusitis mucosa samples and 7 normal ethmoid sinus mucosa samples were used. The protein expression of MUC5AC was examined by using immunohistochemical method. RNAs were extracted from sinus mucosa, and fluorescent quantitative nested RT-PCR was performed for MUC5AC. Results The mean gray scale of MUC5AC protein expression was 159. 72 ± 14. 14 in chronic ethmoid sinusitis mucosa and 115.80 ±31.58 in normal ethmoid sinus mucosa. There was significantly different between two groups (t =3.57, P ＜0.01). The levels of MUC5AC mRNAs in chronic rhinosinusitis (35.80 ± 19. 74) × 105copies/μg] were higher than those in normal sinus mucosa (4. 66 ± 2. 47) × 105 copies/μg] . M UC5 AC mRN A expression had significant difference between chronic ethmoid sinusitis mucosa and normal sinus mucosa(t =4. 12, P ＜0.01). Conlusion This result suggested that up-regulation of MUCSAC in chronic rhinosinusitis might play an important role in the pathogenesis of sinus hyperesecretion in chronic rhinosinusitis.