| 2008年广州手足口病分子流行病学分析 |
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| 引用本文： | 蒋力云,伍业健,耿进妹,陈艺韵,谢华萍,康燕,狄飚,杨智聪,吴新伟.2008年广州手足口病分子流行病学分析[J].预防医学情报杂志,2009,25(10):842-844. |
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| 作者姓名： | 蒋力云 伍业健 耿进妹 陈艺韵 谢华萍 康燕 狄飚 杨智聪 吴新伟 |
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| 作者单位： | 广州市疾病预防控制中心,广州,510080 |
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| 摘 要： | 目的了解引起2008年广州地区手足口病流行的毒株的分子生物流行病学情况。方法首先采用Real-time PCR筛选出总肠道病毒阳性的标本，再分别使用CoxA16和EV71的特异引物进行RT-PCR检测，并对EV71毒株测序，比较序列。结果总肠道病毒阳性率为9．63％，CoxA16阳性率为3．03％，EV71阳性率为2．77％，并归纳出EV71的4个代表性序列。结论广州地区今年流行的EV71毒株并未见大的变异以及毒力加强等情况。
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| 关 键 词： | 手足口病 肠道病毒71型 科萨奇病毒A16型 分子生物学 |
Molecular Epidemiology of Hand-foot-mouth Disease in Guangzhou,2008 |
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| Institution: | JIANG Li-yun, WU Ye-jian, GENG Jin-mei, et al.( Guangzhou Municipal Center for Disease Control and Prevention, Guangzhou 510080, Guangdong Province, China.) |
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| Abstract: | Objective To survey the molecular epidemiology characteristics of the virus strains causing the epidemic of hand-food-mouth disease in Guangzhou during 2008. Method Firstly, Real-time PCR was conducted to select the samples which are enteroviruses positive. Secondly, conventional RT-PCR was conducted with primers of CoxA16 and EV71. Thirdly, EV71 strains were sequenced and analyzed. Results The positive rate of enteroviruses was 9. 63% , of CoxA16 was 3.03%, and of EV71 was 2. 77%. Four representative sequences of EV71 were obtained. Conclusion The prevalent strains of EV71 in Guangzhou in 2008 had no obvious mutation and virulence increasing. |
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| Keywords: | Hand-foot-mouth disease EV71 CoxA16 Molecular biology |
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