Ehrlichia,Coxiella and Bartonella infections in rodents from Guizhou Province,Southwest China |
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| Institution: | 1. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Changping District, Beijing city, China;2. Guizhou Center for Disease Control and Prevention, 550004, Guiyang city, Guizhou province, China;3. Bozhou Center for Disease Control and Prevention, 563100, Zunyi city, Guizhou province, China;4. Liuzhi Center for Disease Control and Prevention, 553400, Liupanshui city, Guizhou province, China;1. Laboratório de Entomologia, Fiocruz Rondônia, Fundação Oswaldo Cruz, Porto Velho, RO, Brazil;2. Programa de Pós-Graduação em Biologia Parasitária, turma especial de Doutorado em Ciências parceria IOC/Fiocruz Rondônia, Porto Velho, RO, Brazil;3. Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal, Universidade Federal de Rondônia, Porto Velho, RO, Brazil;4. Bolsista Fundapam/Laboratório de Biologia do Carrapato, Embrapa Gado de Corte, Campo Grande, MS, Brazil;5. Plataforma de Criação e Experimentação Animal, Fiocruz Rondônia, Fundação Oswaldo Cruz, Porto Velho, RO, Brazil;6. Programa de Pós-Graduação em Biologia Experimental, Universidade Federal de Rondônia, Porto Velho, RO, Brazil;1. Department of Zoology, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, Pakistan;2. Department of Chemistry, Abdul Wali Khan University Mardan, Pakistan;3. Department of Pediatrics, Yale School of medicine Yale University, New Haven, CT, United States;4. Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan;5. Departamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Ñuble, Chile;6. Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, Brazil;1. Laboratoire de Biologie et Ecologie Animales, Département de Biologie Animale - Physiologie Animale, Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso;2. Centre International de Recherche-Développement sur l''Elevage en zone Subhumide (CIRDES), 559, 3-51 Avenue du Gouverneur Louveau, 01 B.P, Bobo-Dioulasso 01 454, Burkina Faso;1. Laboratory of Immunoparasitology, Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, SP, Brazil;2. Laboratory of Parasitic Biology, Environmental Sciences and Farming Sustainability, Dom Bosco Catholic University, Campo Grande 13471-410, MS, Brazil;3. Post Graduation Program of Ecology and Conservation, Mato Grosso do Sul Federal University, Campo Grande, MS, Brazil;1. Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;2. Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil;3. Laboratorio de Moléculas Bioactivas, Departamento de Ciencias Biológicas, CENUR Litoral Norte, Universidad de la República, Paysandú, 60000, Uruguay;4. Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;1. Parasitology Unit, Department of Microbiology and Molecular Genetics, The Kuvin Center for the Study of Infectious and Tropical Diseases, The Hebrew University-Hadassah Medical School, P.O.Box 12272, Jerusalem 91120, Israel;2. Department of Food Processing, Alaca Avni Celik Vocational School, Hitit University, Corum, Turkey;3. Department of Nutrition and Dietetics, Faculty of Health Science, Hitit University, Corum, Turkey;4. Department of Medical Microbiology, Hitit University, Corum, Turkey;5. Department of Nursing, Faculty of Health Science, Hitit University, Corum, Turkey;6. Department of Biology, Faculty of Science and Art, Tokat Gaziosmanpa?a University, Tokat, Turkey;7. Department of Medical Microbiology, Faculty of Medicine, TOBB University of Economics and Technology, Ankara, Turkey |
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| Abstract: | Rodents are generally recognized to be the reservoir hosts of a great many zoonotic pathogens. In some areas of China, rodent-borne pathogens, as well as the role of rodents in the natural cycle of these pathogens, are still poorly investigated. To increase our knowledge on the distribution and epidemiology of rodent-borne bacterial pathogens, 81 rodent liver samples were collected in three locations of Guizhou province located in Southwest China, and screened for the presence of Ehrlichia, Coxiella, and Bartonella in them. A putative novel Ehrlichia species was identified in 5 Berylmys bowersi samples (100%, 5/5). Its 16S rRNA, gltA, and groEL genes have highest 99.84%, 89.11%, and 98.02% identities to those from known Ehrlichia species, and form distinct clades in the phylogenetic trees. Herein we name it “Candidatus Ehrlichia zunyiensis”. Bartonella was tested positive in 8 A. agrarius (striped field mouse), 2 A. chevrieri (Chevrier's field mouse), 1 R. norvegicus (Norway rat), 1 N. confucianus, and 1 N. lotipes, with a total positive rate of 16.05% (13/81). Sequence analysis indicated high genetic diversity in these Bartonella strains. Unexpectedly, two Coxiella strains were identified from the rodents (1 Niviventer confucianus and 1 Mus pahari). Genetic and phylogenetic analysis indicated that both of them are closely related to the Coxiella endosymbiont of ticks. This result supported previous conjectures that vertebrate hosts such as rodents may play a role in the preservation and transmission of Coxiella endosymbiont of ticks. |
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