谷氨酰胺缺乏对∪937、k562细胞生长的抑制

为探索利用白血病细胞和正常细胞氨基酸代谢的差异治疗白血病，本文观察了谷氨酰胺缺乏对∪９３７和Ｋ５６２细胞生长的影响，发现其生长分别被抑制了９２％和８８％，相同条件对ＰＨＡ刺激正常人淋巴细胞抑制率仅１８％，羞异均有非常显著性意义（Ｐ＜０．００１）。谷氨酰胺缺乏对∪９３７和Ｋ５６２细胞ＤＮＡ合成抑制率分别为２０％和６６％，对人正常骨髓单核细胞ＤＮＡ抑制率为５％，差异均有非常显著性意义（Ｐ＜０．０１）；相同条件对这三种细胞ＲＮＡ合成抑制率分别为２９％、２４％和６％，差异亦均有非常显著性意义（Ｐ＜０．０１）。提示可通过剥夺谷氨酰胺治疗急性非淋巴细胞白血病。

THE GROWTH INHIBITION OF ∪937 CELL AND K562 CELL IN GLUTAMINE DEFICIENCY

he growth of ∪937 and K562 cell lines under the condition of gluta-mine deficiency was studied,they were inhibited by 92%and 88%;whilethe growth of PHA-stimulating normal human lyinph cell was inhibitedby 18%,the differences were very significant(P<0.001) DNA synthesesof ∪937,K562 and human normal bone marrow mononuclear cell growthwere inhibited by 20%,66%and 5%respectively,RNA synthesis wereinhibited by 29%,24%and 6%respectively. The differences were verysignificant(P<0.001).The results implicated that nonlymph cell leukemiamight be treated by glutamine deficiency