迷迭香对老化小鼠学习记忆功能及抗氧化能力的影响

目的探究不同剂量迷迭香对老化小鼠(senescence-accelerated mouse,SAM)抗氧化能力的影响。方法采用SAM模型,在基础饲料中添加不同剂量(40,80,120 mg/kg bw)迷迭香提取物。实验8w后,用旷场行为学实验检测小鼠在新异环境中的自发行为,并采用Morris水迷宫观察其学习记忆的行为学变化;并于动物处死后,检测小鼠肝、脑组织MDA含量,SOD、GSH-Px的活力。结果不同剂量(低、中、高)迷迭香组均可显著增加SAM鼠在新异环境中的自发活动和探究行为,Morris水迷宫结果显示,迷迭香组能使SAM小鼠平均潜伏期明显缩短,穿越平台次数显著增加;与模型组相比,迷迭香各组均可显著提高SAM小鼠肝、脑组织中SOD、GSH-Px的活力,明显降低MDA含量。结论迷迭香可明显改善SAM小鼠的学习记忆功能,提高抗氧化能力。营养学报,2012,34(4):384-387]

EFFECT OF ROSEMARY ON THE FUNCTION OF LEARNING AND MEMORY AND ANTIOXIDANT ABILITIES IN SENESCENCE ACCELERATED MICE

Objective To explore the effect of rosemary on the function of learning,memory and antioxidation in senescence-accelerated mice(SAM).Method The SAM were given rosemary at different doses(40,80,120 mg/kg bw).After 8 w,the spontaneous behavior of mice was tested using open field and the learning and memory ability was determined using the Morris water maze tests.Moreover,malondialdehyde(MDA) content,superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activities in brain and liver were also assayed.Results The spontaneous activity in the new environment was significantly increased in different rosemary doses groups in the SAM.The Morris water maze test showed that the average latency period of searching platform was significantly reduced and the times of passing through platform was significantly increased in the rosemary groups.Compared with the model group,the contents of MDA of the rosemary groups were significantly decreased,and the activities of SOD and GSH-Px were significantly increased in brain and liver.Conclusion Rosemary can obviously improve the antioxidant activity and the function of learning and memory in SAM.