黏附分子CD44在呼吸道合胞病毒感染致毛细支气管炎中的作用

目的 探讨黏附分子CD44在呼吸道合胞病毒（RSV）感染致毛细支气管炎中的作用.方法 选择36例RSV感染致毛细支气管炎患儿（感染A组）、34例RSV感染未致毛细支气管炎患儿（感染B组）及同期40例健康儿童（对照组）为研究对象,采用流式细胞仪检测外周血CD44＋淋巴细胞占总淋巴细胞的比例,用酶联免疫吸附法检测各组外周血干扰素（IFN）-γ、白细胞介素（IL）-4、IL-10及IL-12水平,并进行比较分析.结果 感染A组外周血CD44表达水平及IL-4水平高于感染B组及对照组,差异有统计学意义（P＜0.05）.感染A组外周血IFN-γ水平显著低于感染B组及对照组,差异有统计学意义（P＜0.05）.感染A组及感染B组外周血IL-12水平均低于对照组,差异有统计学意义（P＜0.05）.三组外周血IL-10水平比较差异均无统计学意义（P＞0.05）.RSV感染患儿外周血CD44表达水平与IL-4水平呈正相关（r=0.798,P＜ 0.05）,IL-12水平与IL-4水平呈负相关（r=-0.186,P＜0.05）.结论 黏附分子CD44在RSV感染引起的毛细支气管炎中起一定作用,高表达CD44的患儿在感染RSV后患细支气管炎的易感性可能增加.

Role of adhesion molecule CD44 in respiratory syncytial virus infection induced bronchiolitis

Objective To investigate the role ot adhesion molecule CD44 in respiratory syncytial virus （RSV） infection induced bronchiolitis.Methods Thirty-six children with RSV infection induced bronchiolitis （infection A group）,34 children with only RSV infection （infection B group） and 40 healthy children （control group） were selected.The proportion of peripheral blood CD44＋ cell was determined by flow cytometry.The levels of peripheral blood interferon （IFN）-γ,interleukin （IL）-4,IL-10 and IL-12 were determined by enzyme-linked immunosorbent assay.Results The expression of CD44 and the level of IL-4 in peripheral blood in infection A group were significantly higher than those in infection B group and control group,there were statistical differences （P 〈 0.05）.The level of IFN-γ in peripheral blood in infection A group was significantly lower than that in infection B group and control group,there were statistical differences （P 〈0.05）.The levels of IL-12 in peripheral blood in infection A group and infection B group were significantly lower than that in control group,there were statistical differences （P 〈 0.05）.There were no statistical differences in the level of IL-10 in peripheral blood among the 3 groups （P 〉 0.05）.In the children with RSV infection,the expression of CD44 in peripheral blood was positively correlated with the level of IL-4 （r =0.798,P 〈 0.05）,the level of IL-12 was negatively correlated with the level of IL-4 （r =-0.186,P 〈0.05）.Conclusion The expression of adhesion molecule CD44 may play a role in the RSV infection induced bronchiolitis,and the high expression of CD44 in children with RSV infection may increase the susceptibility to bronchiolitis.