| CCK-8法与MTT法检测兔成纤维细胞活性的比较研究 |
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| 引用本文： | 刘爱旗,夏璐.CCK-8法与MTT法检测兔成纤维细胞活性的比较研究[J].现代保健,2013(2):12-13. |
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| 作者姓名： | 刘爱旗 夏璐 |
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| 作者单位： | [1]大连大学,辽宁大连116000 [2]大连大学附属中山医院,辽宁大连116000 |
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| 摘 要： | 目的：探讨CCK-8法和MTT法检测兔成纤维细胞活性的最佳实验条件，并比较其优略。方法；以传至第3代的兔成纤维细胞为研究对象，分别用CCK-8法和MTT法检测兔成纤维细胞的增殖活性。结果：MTT法的最佳检测波长490nm，CCK-8法最佳检测波长为450nm，两法的最佳检测时间均为加入试剂后4h。适宜检测的细胞数量范围为2×10^3／ml-1×10^5／ml个。结论：CCK-8法较MTT法检测的灵敏度高，准确性好，是一种优于MTT法的检测兔成纤维细胞增殖活性的方法。
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| 关 键 词： | CCK-8 MTT 兔成纤维细胞 细胞增殖 |
Comparative Study on the Optimal Experiment Conditions between CCK-8 and MTT in Rabbit Fibroblasts |
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| Authors: | LIU Ai-qi XIA Lu |
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| Institution: | (Dalian University, Dalian 116000, China) |
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| Abstract: | Objective： To investigate optive experiment conditions between MTT and CCK-8 in Rabbit fibroblasts, and to compare which was better.Method： Rabbit fibroblasts of PC3 cell were studied.The activity of rabbit fibroblast was detected respectively with CCK-8 and MTT.Result： For MTT assay, the best wavelength was 490 nm; and the best wavelength of CCK-8 assay was 450 nm.The best test both were 4 h after adding reagent, the number of detect Rabbit fibroblasts of PC3 cell was within the range of 2 × 10^3/ml-1 x 105/mi.Conclusion： CCK-8 assay is better than MTT assay in the aspects of detection sensitivity and accuracy, and CCK-8 is superior to MTT in Rabbit fibroblasts proliferation assay. |
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| Keywords: | CCK-8assy MTT assay Rabbit fibroblasts Proliferation |
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