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四川省HIV- 1 流行毒株的基因序列测定和亚型分析
引用本文:秦光明,邵一鸣,刘刚,苏玲,张灵麟,管永军,郑国英,陈钧,马义才,赵全壁,Josef Kostler,Hans Wolf.四川省HIV- 1 流行毒株的基因序列测定和亚型分析[J].中华流行病学杂志,1998,19(1):39-42.
作者姓名:秦光明  邵一鸣  刘刚  苏玲  张灵麟  管永军  郑国英  陈钧  马义才  赵全壁  Josef Kostler  Hans Wolf
作者单位: 
摘    要:使用PCR对 9份采集于 1996年中旬的四川省经血液途径感染的HIV - 1阳性感染者外周血单个核细胞 (PBMCs)样品进行扩增 ,获得HIV - 1膜蛋白 (env)基因的核酸片段 ,并对其C2-V3及邻区 350~ 4 50个核苷酸序列进行了测定和分析。结果表明 ,9份样品中存在B和C两种亚型的HIV - 1毒株序列 ,其中B亚型 5份 ,彼此间的基因离散率为 1.4 % ;C亚型 4份 ,在不包括sc3号样品的情况下彼此间的基因离散率为 2 .6% ,而sc3号样品与其它 3份样品在核苷酸序列上的差异却达 11.5%。与A~E参考亚型及部分B和C亚型代表株序列相比较 ,属四川B亚型的 5个毒株与包括泰国、缅甸及中国云南德宏在内的B亚型毒株序列十分接近 ,基因离散率在 3.6%~4 .3%的范围内 ;属四川C亚型的 4个毒株除sc3外则与代表印度毒株的C亚型共享序列及德宏C亚型毒株序列十分相似 ,其基因离散率均为 3.1% ,与非洲C亚型毒株nof的基因离散率为13.1% ,而sc3号序列与印度、中国德宏和非洲C亚型HIV - 1的基因离散率却在 10 .1%~ 17.2 %的范围内。提示 ,HIV - 1在四川的流行时间不长 ,且其B和除sc3之外的C亚型毒株的传入与流行在云南德宏州的相同亚型HIV - 1毒株密切相关 ,而由sc3所代表的C亚型毒株则存在目前还尚不了解的其它传入来源。

关 键 词:人免疫缺陷病毒I型  亚型  基因变异

Subtype and Sequence Analysis of the C2- V3 Region of gp120 Genes among HIV-1 Strains in Sichuan Province
Qin Guangming,Shao Yiming,Liu Gang.Subtype and Sequence Analysis of the C2- V3 Region of gp120 Genes among HIV-1 Strains in Sichuan Province[J].Chinese Journal of Epidemiology,1998,19(1):39-42.
Authors:Qin Guangming  Shao Yiming  Liu Gang
Institution:Sichuan Sanitation and Anti-epidemic Station, Chengdu.
Abstract:Nine DNA fragments of HIV 1 env gene were amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from HIV 1 sero positive intravenous drug users (IDUs) in Sichuan province,in mid 1996.Their C2 V3 region (about 450 bp) was sequenced.Sequence analysis showed that there were two HIV-1 subtypes B and C,with 1.4% and 2.6% gene divergence inside each subtype,in Sichuan.The 5 subtype B strains,when comparing with sequences of several reference HIV-1 strains,were found similar to those identified in Thailand,Myanma and Yunnan province.The nucleotide sequence divergence between them was ranged from 3.6% to 4.3%. Mean while,the 4 subtype C strains except sc3,were most close to those found in India as well as in Yunnan province,and both their nucleotide sequence divergence was 3.1%.The sc3 strain belonged to subtype C but had larger genetic distance,between 10.1%-17.2%.Above results suggested a recent epidemic of both subtype HIV-1 strains in Sichuan provine and they were very close to those identified in Yunnan province.The subtype C strain of sc3 is special and its source remains notclear.
Keywords:HIV-1    Subtype    Gene variation
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