首页 | 本学科首页   官方微博 | 高级检索  
检索        

湖北省HIV-1流行毒株的基因序列测定和亚型分析
引用本文:李允文,邵一鸣,罗小光,苏玲,张流波,陈均,方越,袁健中,张艺,陈惠萍,Josef Kostler,Hans Wolf.湖北省HIV-1流行毒株的基因序列测定和亚型分析[J].中华流行病学杂志,1997,18(4):217-219.
作者姓名:李允文  邵一鸣  罗小光  苏玲  张流波  陈均  方越  袁健中  张艺  陈惠萍  Josef Kostler  Hans Wolf
作者单位:湖北省卫生防疫站,中国预防医学科学院病毒所艾滋病参比实验室,德国累根斯堡大学医学微生物学研究所
摘    要:1996年8月下旬采集20份湖北省HIV1感染者外周血,分离单个核细胞(PBMCs)提取核酸使用PCR进行扩增获得HIV1膜蛋白(env)基因的核酸片段,并对其C2V3及邻区350~450个核苷酸序列进行了测定和分析。研究结果表明,这20份样品均为B亚型的HIV1毒株序列,彼此间的基因离散率为1.9%。与国际AE亚型参考序列及部分地区B亚型代表株序列相比较,湖北的20个毒株与流行与我国云南德宏、四川和河南等地的B亚型毒株序列十分接近,均属于泰国B亚型;它们的基因离散率在1.9%~3.2%的范围内。根据以上数据及其它资料提示,HIV1在湖北的流行时间不长,该流行区毒株的传入与流行在云南德宏州的相同亚型HIV1毒株密切相关。

关 键 词:HIV-1  亚型  基因变异  序列测定
收稿时间:1997/3/3 0:00:00
修稿时间:1997/4/7 0:00:00

Subtype and Sequence Analysis of the C2-V3 Region of gp120 Genes among HIV-1 Strains in Hubei Province
Li Yun-wen,Shao Yi-ming,Luo Xiao-guang.Subtype and Sequence Analysis of the C2-V3 Region of gp120 Genes among HIV-1 Strains in Hubei Province[J].Chinese Journal of Epidemiology,1997,18(4):217-219.
Authors:Li Yun-wen  Shao Yi-ming  Luo Xiao-guang
Institution:Li Yun wen *,Shao Yi ming,Luo Xiao guang,et al.* Hubei Sanitation and Anti epidemic Station,Wuhan 430070
Abstract:Twenty DNA fragments of HIV 1 env gene were amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from HIV 1 sero positive people from Hubei province in the end of 1996.The C2 V3 region (about 450 bp) was sequenced.Sequence analyses showed that all of them were HIV 1 subtypes,B strain,with 1.9% gene divergence innergroup.The 20 subtype B strains,compared with sequences of several reference HIV 1 strains,were closely related to those found in Thailand,Myanma and Yunnan and other provinces in China.The nucleotide sequence divergence between them ranged from 1.9% to 3.2%. These results suggested that recent epidemic of B subtype HIV 1 strains in Hubei province and the responsible virus were very close to those identified in Yunnan province.
Keywords:HIV  1  Subtype  Gene variation  Sequencing
本文献已被 CNKI 维普 等数据库收录!
点击此处可从《中华流行病学杂志》浏览原始摘要信息
点击此处可从《中华流行病学杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号