宫颈癌危险因素的流行病学研究

宫颈癌病因复杂，多数研究者认为是多因素综合作用的结果。本文通过病例－对照研究，应用分子及血清流行病学方法，探讨引起宫颈癌的危险因素。应用ＨＰＶ６／１１、ＨＰＶ１６／１８探针，通过斑点分子杂交技术，检测宫颈癌和对照宫颈组织ＤＮＡ中的感染，结果病例组和对照组ＨＰＶ６／１１阳性率分别为２４．６％、２７．５％，差别无显著性，ＨＰｙ１６／１８阳性率分别为４５．６％、９．８％，差别有显著性；应用ＰＣＲ技术检测两组宫颈组织ＤＮＡ中ＨＳＶ－２感染，结果病例组和对照组阳性率分别为２８．１％、７．８％，Ｌｏｇｉｓｔｉｃ回归平衡年龄因素后差别无显著性；应用ＥＬＩＳＡ方法检测血清中ＨＣＭＶ抗体阳性率，结果病例组和对照组ＩｇＭ阳性率分别为１２．３％、５．９％，差别无显著性。用多因素Ｌｏｇｉｓｔｉｃ回归平衡各因素间的作用，最后分析结果表明，文化程度、产次、ＨＰＶ１０／１８感染与宫颈癌的发生有关，其ＯＲ值分别为０．５７、１．８５、３１．９６。

Epidemiological Study on Risk Factors of Cervical Cancer

he relationship between cervical cancer and risk factors including virus infection of cervix was studied with methods of molecalar and serological epidemiology.DNA extracted from cervical biopsies taken from 57 cases and 51 controls were tested for HPV6/11,HPV16/18 by Dot-blot hybridization and for HSV-2 by PCR.Sera were detected for HCMV-IgM by ELISA.The results and risk factors were ana- lyzed by logistic regression model.The multiple logistic regression model re- sults showed that HPV16/18 infection was animportant risk factor,the infection rate was significantly higher im the group of cervicalcancer,the OR was 31.96.Other risk factorswere the educational level and the number ofbirth,the OR were 0.57 and 1.85,respectively.the difference of HSV-2 infection of cervixand HCNV-IgM in serum was not significantbetween the two groups.