|
|||||
|
|
| 黑龙江林区野鼠伯氏疏螺旋体核酸检测与序列分析 | |
| 引用本文： | 左双燕,唐琨,李颖,于季红,张圆,倪雪冰,郑元春,霍秋波,宋玉东,曾小敏.黑龙江林区野鼠伯氏疏螺旋体核酸检测与序列分析[J].中华流行病学杂志,2012,33(6):643-644. |
| 作者姓名： | 左双燕 唐琨 李颖 于季红 张圆 倪雪冰 郑元春 霍秋波 宋玉东 曾小敏 |
| 作者单位： | 1. 中南大学公共卫生学院流行病与卫生统计学系,长沙,410078 2. 苏州大学城市学院 3. 解放军总医院感染与控制科 4. 黑龙江省牡丹江林业中心医院 |
| 基金项目： | 国家重点基础研究发展计划(973计划)，国家杰出青年基金 |
| 摘 要： | 伯氏疏螺旋体共有13个基因种,国内分离到的菌株包括5个基因种1-3],本研究对黑龙江地区啮齿动物中伯氏疏螺旋体进行分子流行病学调查和分析.
1.材料与方法:
(1)样本采集、处理及DNA提取:2009-2011年4-7月从黑龙江林区采用夹夜法采集野鼠,每样点100×2夹次,按5m距离布放鼠夹,晚放晨收.现场鉴定鼠种后,将捕获的鼠放入鼠袋内,乙醚麻醉后,消毒,无菌取脾脏低温保存.采用天根生化科技(北京)有限公司血液/细胞/组织基因组DNA提取试剂盒提取脾脏DNA,-20℃贮存备用. |
| 关 键 词： | 伯氏疏螺旋体 序列分析 |
| 收稿时间： | 2011/11/28 0:00:00 |
DNA detection and sequence analysis of Borrelia burgdorferi sensu lato in rodents from Helongjiang forest region |
|
| ZUO Shuang-yan,TANG Kun,LI Ying,YU Ji-hong,ZHANG Yuan,NI Xue-bing,ZHENG Yuan-chun,HUO Qiu-bo,SONG Yu-dong and ZENG Xiao-min.DNA detection and sequence analysis of Borrelia burgdorferi sensu lato in rodents from Helongjiang forest region[J].Chinese Journal of Epidemiology,2012,33(6):643-644. | |
| Authors: | ZUO Shuang-yan TANG Kun LI Ying YU Ji-hong ZHANG Yuan NI Xue-bing ZHENG Yuan-chun HUO Qiu-bo SONG Yu-dong and ZENG Xiao-min |
| Institution: | Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China;Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China;School of Architecture and Urban Environment, Soochow University;Department of Infection Management and Disease Control, General Hospital of the People''s Liberation Army;Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China;Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China;Forestry Center Hospital, Mudanjiang City, Helongjiang Province;Forestry Center Hospital, Mudanjiang City, Helongjiang Province;Forestry Center Hospital, Mudanjiang City, Helongjiang Province;Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078, China |
| Abstract: | |
| Keywords: | Borrelia burgdorferi sensu lato Sequence analysis |
| 本文献已被 万方数据 等数据库收录！ | |
| 点击此处可从《中华流行病学杂志》浏览原始摘要信息 | |
| 点击此处可从《中华流行病学杂志》下载免费的PDF全文 | |