浙江省啮齿动物中温州病毒的基因组学分析

目的 为了解国内发现的新型沙粒病毒（温州病毒）在温州地区啮齿类动物中的流行情况。方法 采用巢式RT-PCR方法分别对采集自温州（48份）及龙泉（156份）的啮齿动物样本筛查沙粒病毒。结果 在温州地区采集的5份啮齿动物样本中再次检测到温州病毒，阳性率为10.41%；但在龙泉地区采集的156份样本中均未发现温州病毒。分析病毒的全基因组序列发现其中4株病毒与已知的温州病毒有很高的同源性；而编号为Wencheng-Rn-288病毒株的S和L节段与已知病毒同源性分别为87.5%和91.6%，在进化树上为一独立分支，为温州病毒的新亚型。结论 温州病毒在温州地区啮齿动物中有较高的流行率，且呈多样性。鉴于在柬埔寨已发现温州病毒能引起人呼吸道疾病，因此我国应加强温州病毒的监测。

Genomic analysis of Wenzhou virus in rodents from Zhejiang province

Objective Arenavirus is a negative single-stranded RNA virus and an important human pathogen, mainly harbored and transmitted by rodents, causing severe diseases, including hemorrhagic fever and encephalitis. Following the discovery of a novel pathogenic arenavirus (Wenzhou virus, WENV), the prevalence of WENV in local small rodents was investigated. Methods By using RT-PCR, WENV was screened in 48 and 156 rodents sampled from Wenzhou and Longquan, respectively. Results Consequently, WENV was detected in 5 (10.41%) rodents sampled from Wenzhou. However, no WENV was identified in all the rodents sampled from Longquan. Genetic analysis of complete genome sequences indicated that 4 of 5 virus strains were closely related to the known Wenzhou viruses with high homology. Especially, the L and S segments of Wencheng-Rn-288 strain shared homology of 87.5% and 91.6% with other viruses, respectively. They formed a distinct lineage, suggesting that this strain might be a novel variant of WENV. Conclusions Our results indicate that WENV has a high prevalence and high genetic diversity among rodents in Wenzhou. As the respiratory disease caused by WENV has been detected in Cambodia, it is necessary to strengthen the surveillance for WENV in China.