应用离子色谱法快速诊断厌氧菌感染

本实验对临床采集的51份标本,以常规的厌氧菌分离方法,与使用离子色谱仪直接分析标本的方法相对照,结果检出异丁酸、丁酸、异戊酸、戊酸的阳性相关率为93.5%。其中异丁酸和异戊酸与拟杆菌属检出相关性达94%。异丁酸、丁酸与梭杆菌属和梭菌属的阳性相关性为92~93%。使用离子色谱仪直接分析临床标本,对于厌氧菌感染症的快速推断性诊断有肯定价值,可推荐临床检验中试用。

Rapid Diagnosis of Anaerobic Infections with the Aid of lon-Chromatography

In studying 51 clinical specimens for anaerobes, we compared the results of using the usual methods of bacterial isolation with those by applying directly the technique of ion-chromatography (IC) on the specimens. It was found that the correlation between the presence of isobutyl acid, butyric acid, isovaleric acid, valeric acid and positive culture of anaerobes was 93.5%, the correlation between the Presence of isobuty lacid, isovaleric acid and that of Bacterial isolation was 94%; the correlation between the presence of isobutyl acid, butyric acid or valerid acid and the recovery of Fusobacterium and Clostridium was 92-93%. It is our opinion that IC technique applied directly to the specimens is of value in revealing the presence of anaerobic infections, and it could be used in assisting the laboratory diagnosis clinically.