| 蚊虫携带登革病毒快速筛查方法的建立 |
| |
| 引用本文： | 宋锋林,王静,周蕾,吴刚,薛芳,张晓龙,姚李四,杜海方,张冠楠.蚊虫携带登革病毒快速筛查方法的建立[J].中国国境卫生检疫杂志,2009(2):118-120. |
| |
| 作者姓名： | 宋锋林 王静 周蕾 吴刚 薛芳 张晓龙 姚李四 杜海方 张冠楠 |
| |
| 作者单位： | 辽宁出入境检验检疫局;中国检验检疫科学院;军事医学科学院微生物流行病研究所; |
| |
| 基金项目： | “十一五”国家科技支撑计划项目(2006BAK10B07);;国家质检总局科研项目(2007IK190) |
| |
| 摘 要： | 目的]研究一种适合现场使用的快速检测方法，用于对蚊虫携带登革病毒的初步筛查。方法]采用双抗体夹心技术，建立上转换发光（UCP）免疫层析方法，筛查蚊体携带的登革病毒。结果]该方法能在15min内对蚊虫携带的登革病毒进行快速筛查。结论]建立的免疫层析方法简便、快速、敏感、特异，适合卫生检疫工作现场使用。
|
| 关 键 词： | 蚊 登革病毒 免疫层析 上转换发光（UCP） |
A Methodology Development for Rapid Detecting Dengue Virus Carried by Mosquito |
| |
| Song Fenglin,Wang Jing,Zhou Lei,Wu Gang,Xue Fang,Zhang Xiaolong,Yao Lisi,Du Haifang,Zhang Guannan..A Methodology Development for Rapid Detecting Dengue Virus Carried by Mosquito[J].Chinese Journal of Frontier Health and Quarantine,2009(2):118-120. |
| |
| Authors: | Song Fenglin Wang Jing Zhou Lei Wu Gang Xue Fang Zhang Xiaolong Yao Lisi Du Haifang Zhang Guannan |
| |
| Institution: | 1. Liaoning Entry-Exit Inspection and Quarantine Bureau;Dalian 116001;China;2. Chinese Academy of Inspection and Quarantine;Beijing 100025;3. Institute of Microbiology and Epidemiology;The Academy of Military Medical Sciences;Beijing 100071;China |
| |
| Abstract: | Objective To develop a method for rapid detecting dengue virus carried by mosquito. Method Up-converting Phosphor (UCP) immunity chromatography assay was used based on double antibody sandwiched assay to detect dengue virus carried by mosquito. Result It took 15 minutes to detect dengue virus carried by mosquito by UCP immunity chromatography assay. Conclusion The UCP immunity chromatography assay proved to be a rapid, convenient, specific and sensitive test for detecting dengue virus carried by mosquito. |
| |
| Keywords: | Mosquito Dengue virus Immunity chromatography Up-converting Phosphor （UCP） |
| 本文献已被 CNKI 维普 等数据库收录！ |
