The effect of Mycobacterium tuberculosis CRISPR-associated Cas2 (Rv2816c) on stress response genes expression,morphology and macrophage survival of Mycobacterium smegmatis |
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| Affiliation: | 1. Department of Pathology and Molecular Medicine, Queen''s University, Kingston, Ontario, Canada;2. INCT do Sangue Hemocentro UNICAMP, INCT do Sangue Hemocentro UNICAMP, University of Campinas, Campinas, Brazil;3. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91125;4. Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109;5. Department of Immunology, University of Connecticut School of Medicine, Farmington, Connecticut 06030;6. Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784, South Korea;1. Health Care Department of South Building, Chinese People''s Liberation Army General Hospital, Beijing, China;2. National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China;3. Liaoning Provincial Center for Disease Control and Prevention, Shenyang, China;4. Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children''s Hospital, Capital Medical University, Beijing, China;1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China;2. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China |
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| Abstract: | Clustered regularly interspaced short palindromic repeats (CRISPR) are present in the genome of 40% bacteria and 90% archaea. CRISPR and accompanying Cas proteins constitute an adaptive immune system against disruptive mobile genetic elements. Two CRISPRs and 9 genes encoding CRISPR-associated proteins have been found in the genome of Mycobacterium tuberculosis. The CRISPR-associated Cas2 is an endoribonuclease required for the acquisition of new spacers. In this study, Cas2 encoded by Rv2816c was expressed in Mycobacterium smegmatis lacking CRISPR-Cas system and its role in stress responses of M. smegmatis in vitro and within macrophages was studied. We found that Cas2 mediated M. smegmatis stress response changes were associated with the altered expression of sigma factors which involved in mycobacterial stress response and virulence. We also found that Cas2 decreased the survival of M. smegmatis within macrophages. This study provides new insights on the role of Cas2. |
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